公开(公告)号：US20120079611A1

公开(公告)日：2012-03-29

申请号：US13240117

申请日：2011-09-22

申请人： David Q. Shih ,  Stephen R. Targan

发明人： David Q. Shih ,  Stephen R. Targan

IPC分类号： A01K67/027 ,  C12N5/10 ,  G01N33/48 ,  C12N15/85

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0368 ,  A01K2267/0387 ,  A01K2267/0393 ,  A61K49/0008 ,  C07K14/4705

摘要： This invention relates transgenic animals that overexpress TL1A in a tissue specific manner to model inflammatory bowel disease (IBM such as colitis, Crohn's disease and ulcerative colitis, fibrosis, and related inflammatory diseases and conditions. TL1A transgenic animals constitutively express both TL1A and GFP in lymphoid and myeloid cell lineages, allowing convenient identification and sorting of immune cells involved in IBD disease progression, such as T-cells, antigen presenting cells (APC), and dendritic cells (DC). TL1A transgenic animals may be induced to exhibit gross fibrosis, or isolated cells may be implanted into immunodeficient mice to establish colitis.

摘要翻译： 本发明涉及以组织特异性方式过表达TL1A的转基因动物，以模拟炎症性肠病（IBM例如结肠炎，克罗恩病和溃疡性结肠炎，纤维化及相关的炎性疾病和病症）。TL1A转基因动物在淋巴中组成型表达TL1A和GFP 和骨髓细胞谱系，允许方便地鉴定和分选参与IBD疾病进展的免疫细胞，例如T细胞，抗原呈递细胞（APC）和树突状细胞（DC），TL1A转基因动物可能被诱导出显着的纤维化， 或分离的细胞可以植入免疫缺陷小鼠中以建立结肠炎。

公开(公告)号：US08766034B2

公开(公告)日：2014-07-01

申请号：US13240117

申请日：2011-09-22

申请人： David Q. Shih ,  Stephen R. Targan

发明人： David Q. Shih ,  Stephen R. Targan

IPC分类号： A01K67/00

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0368 ,  A01K2267/0387 ,  A01K2267/0393 ,  A61K49/0008 ,  C07K14/4705

摘要： This invention relates transgenic animals that overexpress TL1A in a tissue specific manner to model inflammatory bowel disease (IBM such as colitis, Crohn's disease and ulcerative colitis, fibrosis, and related inflammatory diseases and conditions. TL1A transgenic animals constitutively express both TL1A and GFP in lymphoid and myeloid cell lineages, allowing convenient identification and sorting of immune cells involved in IBD disease progression, such as T-cells, antigen presenting cells (APC), and dendritic cells (DC). TL1A transgenic animals may be induced to exhibit gross fibrosis, or isolated cells may be implanted into immunodeficient mice to establish colitis.

摘要翻译： 本发明涉及以组织特异性方式过表达TL1A的转基因动物，以模拟炎症性肠病（IBM例如结肠炎，克罗恩病和溃疡性结肠炎，纤维化及相关的炎性疾病和病症）。TL1A转基因动物在淋巴中组成型表达TL1A和GFP 和骨髓细胞谱系，允许方便地鉴定和分选参与IBD疾病进展的免疫细胞，如T细胞，抗原呈递细胞（APC）和树突状细胞（DC），TL1A转基因动物可能被诱导出显着的纤维化， 或分离的细胞可以植入免疫缺陷小鼠中以建立结肠炎。

公开(公告)号：US08163501B2

公开(公告)日：2012-04-24

申请号：US12645394

申请日：2009-12-22

申请人： Stephen R. Targan ,  Eric A. Vasiliauskas ,  William S. Mow ,  Huiying Yang ,  Phillip R. Fleshner ,  Jerome I. Rotter

发明人： Stephen R. Targan ,  Eric A. Vasiliauskas ,  William S. Mow ,  Huiying Yang ,  Phillip R. Fleshner ,  Jerome I. Rotter

IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/00

CPC分类号： G01N33/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N33/6893 ,  G01N2469/20 ,  G01N2800/065

摘要： The invention provides a method of diagnosing or predicting susceptibility to a clinical subtype of Crohn's disease in a subject having Crohn's disease by determining the presence or absence of IgA anti-I2 antibodies in the subject, where the presence of the IgA anti-I2 antibodies indicates that the subject has a clinical subtype of Crohn's disease. In one embodiment, a method of the invention is practiced by further determining the presence or absence in the subject of a NOD2 variant, anti-Saccharomyces cerevisiae antibodies (ASCA), IgA anti-OmpC antibodies, or perinuclear anti-neutrophil cytoplasmic antibodies (pANCA). The methods of the invention can be used to diagnose or predict susceptibility to a clinical subtype of Crohn's disease, for example, a fibrostenotic subtype, a subtype characterized by the need for small bowel surgery, or a subtype characterized by the absence of features of ulcerative colitis.

摘要翻译： 本发明提供一种诊断或预测克罗恩病临床亚型易感性的方法，其通过测定受试者中IgA抗-I2抗体的存在或不存在，其中IgA抗-I2抗体的存在表明 该受试者具有克罗恩病的临床亚型。 在一个实施方案中，通过进一步确定受试者中NOD2变体，抗酿酒酵母抗体（ASCA），IgA抗OmpC抗体或核周抗中性粒细胞胞质抗体（pANCA）的存在或不存在来实施本发明的方法 ）。 本发明的方法可以用于诊断或预测对克罗恩病的临床亚型的易感性，例如纤维狭窄亚型，特征在于需要小肠手术的亚型或特征在于不存在溃疡特征的亚型 结肠炎。