公开(公告)号：US5149623A

公开(公告)日：1992-09-22

申请号：US303819

申请日：1989-02-01

Applicant: James R. Carlson ,  Steve C. Mertens ,  Joann L. Yee

Inventor： James R. Carlson ,  Steve C. Mertens ,  Joann L. Yee

IPC: C07K14/16 ,  G01N33/569

CPC classification number: C07K14/005 ,  G01N33/56988 ,  C12N2740/16122 ,  Y10S435/974 ,  Y10S435/975

Abstract: A new dot enzyme immunoassay (EIA) with a conserved portion of the envelope protein of the human immunodeficiency virus (HIV) as antigen has been designed for use in areas with few laboratory facilities and by personnel with little laboratory experience. Sera were tested in 263 subjects who had AIDS or AIDS-related complex or were at -risk or not-at-risk of AIDS from the USA, Africa, and Asia/Oceania. The dot EIA was 100% sensitive in the American subjects, and there were only 2 false negatives in the others, both of which were negative by commerical EIA. The test is simple to perform, economical, rapid (30 min), and stable.

Abstract translation: 具有作为抗原的人类免疫缺陷病毒（HIV）的包膜蛋白的保守部分的新的点酶免疫测定法（EIA）被设计用于具有少量实验室设施的区域和具有很少实验室经验的人员。 在有艾滋病或与艾滋病有关的复合体的263名受试者中进行血清检测，或者来自美国，非洲和亚洲/大洋洲的艾滋病感染者或非艾滋病感染者。 点EIA在美国科目中是100％敏感的，其他的只有2个假阴性，两者都是商业环境影响评估的阴性。 测试执行简单，经济，快速（30分钟），稳定。