公开(公告)号：US20100035300A1

公开(公告)日：2010-02-11

申请号：US11364716

申请日：2006-02-27

申请人： Andrew H.-J. Wang ,  Ting-Fang Wang ,  Yan-Ping Shih ,  Hui-Chung Wu ,  Su-Ming Hu

发明人： Andrew H.-J. Wang ,  Ting-Fang Wang ,  Yan-Ping Shih ,  Hui-Chung Wu ,  Su-Ming Hu

IPC分类号： C12P21/00 ,  C12N1/20

CPC分类号： C12P21/06 ,  C07K2319/50 ,  C12N9/503

摘要： A cis-TEVP fusion protein including a TEVP protease, a TEVP cleavage site and a target protein provide a platform for expression of the target protein. A trans-TEVP fusion protein including a TEVP cleavage site and a target protein, the amino-terminal portion of the target protein adjacent to the C-terminal portion of the TEVP cleavage site, the amino acidic residue in position P2 of the TEVP cleavage site being a Valine also produces the target protein by the same process. A cis-TEVP fusion protein system comprising the first fusion protein and a suitable host cell; a trans-TEVP fusion protein system comprising the second fusion protein and a suitable host cell; associated methods to produce target proteins, and kits of parts are also disclosed herein.

摘要翻译： 包含TEVP蛋白酶，TEVP切割位点和靶蛋白的顺式TEVP融合蛋白提供靶蛋白表达的平台。 包含TEVP切割位点和靶蛋白的TETEP融合蛋白，与TEVP切割位点的C末端部分相邻的靶蛋白的氨基末端部分，TEVP切割位点P2位置的氨基酸残基 作为缬氨酸也通过相同的过程产生靶蛋白。 包含第一融合蛋白和合适宿主细胞的顺-TEVP融合蛋白系统; 包含第二融合蛋白和合适的宿主细胞的反TEVP融合蛋白系统; 本文还公开了产生靶蛋白的相关方法和成分的试剂盒。