利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.011 秒)

    Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
    1.
    发明授权
    Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions 失效
    用于增加体外RNA转录和其他多核苷酸合成反应产率的组合物和方法

    公开(公告)号：US5256555A

    公开(公告)日：1993-10-26

    申请号：US810968

    申请日：1991-12-20

    申请人： Susan C. Milburn ,  Marianna Goldrick ,  Matthew Winkler

    发明人： Susan C. Milburn ,  Marianna Goldrick ,  Matthew Winkler

    IPC分类号： C12P19/34 ,  C12N9/14 ,  C07H15/12

    CPC分类号： C12P19/34

    摘要： The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA transcription and in various other enzymatic reactions in which a polynucleotide is synthesized. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.

    摘要翻译： 本发明涉及增加多核苷酸合成反应产率的组合物和方法。 特别地，本发明涉及用于体外RNA转录的改进的反应混合物和其中合成多核苷酸的各种其它酶反应。 反应混合物使用高浓度的总核苷酸，大约12mM至40mM，即先前认为是抑制性的水平。 还公开了其它有用的修饰。