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18 条记录 (耗时 0.024 秒)

    Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
    2.
    发明授权
    Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions 失效
    用于增加体外RNA转录和其他多核苷酸合成反应产率的组合物和方法

    公开(公告)号：US5256555A

    公开(公告)日：1993-10-26

    申请号：US810968

    申请日：1991-12-20

    申请人： Susan C. Milburn ,  Marianna Goldrick ,  Matthew Winkler

    发明人： Susan C. Milburn ,  Marianna Goldrick ,  Matthew Winkler

    IPC分类号： C12P19/34 ,  C12N9/14 ,  C07H15/12

    CPC分类号： C12P19/34

    摘要： The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA transcription and in various other enzymatic reactions in which a polynucleotide is synthesized. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.

    摘要翻译： 本发明涉及增加多核苷酸合成反应产率的组合物和方法。 特别地，本发明涉及用于体外RNA转录的改进的反应混合物和其中合成多核苷酸的各种其它酶反应。 反应混合物使用高浓度的总核苷酸，大约12mM至40mM，即先前认为是抑制性的水平。 还公开了其它有用的修饰。

    Methods for the recovery of nucleic acids from reaction mixtures
    3.
    发明授权
    Methods for the recovery of nucleic acids from reaction mixtures 失效
    从反应混合物中回收核酸的方法

    公开(公告)号：US5422241A

    公开(公告)日：1995-06-06

    申请号：US724423

    申请日：1991-07-03

    申请人： Marianna Goldrick ,  Matthew Winkler

    发明人： Marianna Goldrick ,  Matthew Winkler

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12N9/22 ,  C12N9/99

    CPC分类号： C12N15/1003 ,  C12Q1/6806

    摘要： A method for the recovery of nucleic acids from a reaction mixture, such as nuclease protection assays, comprising the use of one reagent containing a) nuclease for digesting single-stranded RNA and b) a nucleic acid precipitating carrier agent, (sheared DNA or linear acrylamide). A second reagent contains a chaotropic agent (a guanidinium salt) for inactivating said nucleases and an alcohol (ethanol or isopropanol) for the simultaneous inactivation of the nucleases and the precipitation of the nucleic acids without the need for protease digestion or organic extraction.

    摘要翻译： 用于从反应混合物中回收核酸的方法，例如核酸酶保护测定法，其包括使用含有a）核酸酶以消化单链RNA的一种试剂和b）核酸沉淀载体试剂（剪切DNA或线性 丙烯酰胺）。 第二试剂包含用于灭活所述核酸酶的离液剂（胍盐）和用于同时灭活核酸酶和沉淀核酸而不需要蛋白酶消化或有机提取的醇（乙醇或异丙醇）。

    Compositions and methods for increasing the yields of the in vitro RNA transcription and other polynucleotide synthetic reactions
    5.
    发明授权
    Compositions and methods for increasing the yields of the in vitro RNA transcription and other polynucleotide synthetic reactions 失效
    用于增加体外RNA转录和其他多核苷酸合成反应产率的组合物和方法

    公开(公告)号：US06586219B2

    公开(公告)日：2003-07-01

    申请号：US09966494

    申请日：2001-09-28

    申请人： Susan Milburn ,  Marianna Goldrick ,  Matthew Winkler

    发明人： Susan Milburn ,  Marianna Goldrick ,  Matthew Winkler

    IPC分类号： C12N914

    CPC分类号： C12P19/34

    摘要： The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA trancription and in various other enzymatic reactions in which a polynucleotide is synthesized. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.

    摘要翻译： 本发明涉及增加多核苷酸合成反应产率的组合物和方法。 特别地，本发明涉及用于体外RNA转录和其中合成多核苷酸的各种其它酶反应中的改进的反应混合物。 反应混合物使用高浓度的总核苷酸，大约12mM至40mM，即先前认为是抑制性的水平。 还公开了其它有用的修饰。

    Stripping nucleic acids with iodine and sodium thiosulfate
    7.
    发明授权
    Stripping nucleic acids with iodine and sodium thiosulfate 失效
    用碘和硫代硫酸钠剥离核酸

    公开(公告)号：US06365731B1

    公开(公告)日：2002-04-02

    申请号：US08907195

    申请日：1997-08-06

    申请人： David Brown ,  Matthew Winkler

    发明人： David Brown ,  Matthew Winkler

    IPC分类号： C07H2100

    CPC分类号： C12Q1/6834 ,  Y10S435/975 ,  C12Q2523/107

    摘要： The present invention is directed to compositions and methods for removal of nucleic acid probes from sample nucleic acids, particularly when the sample nucleic acids are attached to a solid support. The invention also concerns methods of stripping and reusing nucleic acid blots.

    摘要翻译： 本发明涉及从样品核酸中去除核酸探针的组合物和方法，特别是当样品核酸连接到固体支持物上时。 本发明还涉及剥离和重复使用核酸印迹的方法。

    Methods and compositions for stripping nucleic acids
    9.
    发明授权
    Methods and compositions for stripping nucleic acids 失效
    剥离核酸的方法和组合物

    公开(公告)号：US06891032B2

    公开(公告)日：2005-05-10

    申请号：US10082254

    申请日：2002-02-25

    申请人： David Brown ,  Matthew Winkler

    发明人： David Brown ,  Matthew Winkler

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12Q1/6834 ,  Y10S435/975 ,  C12Q2523/107

    摘要： The present invention is directed to compositions and methods for removal of nucleic acid probes from sample nucleic acids, particularly when the sample nucleic acids are attached to a solid support. The invention also concerns methods of stripping and reusing nucleic acid blots.

    摘要翻译： 本发明涉及从样品核酸中去除核酸探针的组合物和方法，特别是当样品核酸连接到固体支持物上时。 本发明还涉及剥离和重复使用核酸印迹的方法。