公开(公告)号：US06986990B1

公开(公告)日：2006-01-17

申请号：US10019832

申请日：2000-04-26

申请人： Takeshi Nagasu ,  Yuji Sugita ,  Tomoko Fujishima ,  Tadahiro Oshida ,  Masaya Obayashi ,  Shigemichi Gunji ,  Izumi Obayashi ,  Yukiho Imai ,  Nei Yoshida ,  Kaoru Ogawa ,  Keiko Matsui

发明人： Takeshi Nagasu ,  Yuji Sugita ,  Tomoko Fujishima ,  Tadahiro Oshida ,  Masaya Obayashi ,  Shigemichi Gunji ,  Izumi Obayashi ,  Yukiho Imai ,  Nei Yoshida ,  Kaoru Ogawa ,  Keiko Matsui

IPC分类号： C12Q1/66

CPC分类号： C07K14/47

摘要： T cells from multiple subjects who showed different cedar pollen specific IgE titers were prepared before and after cedar pollen dispersal, and differential display analysis was performed to screen for pollen antigen responsive genes. As a result, a novel gene was successfully isolated whose expression is significantly higher in subjects with high cedar pollen specific IgE titer. The inventors have found that it is possible to use this gene to test for an allergic disease and to screen for candidate compounds for therapeutic drugs for an allergic disease.

摘要翻译： 在雪松花粉散布前后制备了显示不同雪松花粉特异性IgE滴度的多个受试者的T细胞，进行差异显示分析筛选花粉抗原应答基因。 结果，成功地分离出一种新型基因，其表达在具有高雪松花粉特异性IgE滴度的受试者中显着更高。 发明人已经发现，可以使用该基因来测试过敏性疾病并筛选用于过敏性疾病的治疗药物的候选化合物。

公开(公告)号：US06511816B1

公开(公告)日：2003-01-28

申请号：US09306454

申请日：1999-05-06

申请人： Akira Nakanishi ,  Tadahiro Oshida ,  Tadahiro Matsushita ,  Tetsuo Onuki

发明人： Akira Nakanishi ,  Tadahiro Oshida ,  Tadahiro Matsushita ,  Tetsuo Onuki

IPC分类号： C12Q100

CPC分类号： C07K14/245 ,  C07K14/25

摘要： The present invention discloses a protein that possesses an ability to inhibit bacterial DNA gyrase activity (DNA Gyrase-inhibiting protein; DGI); a host cell transformed by a replicable expression vector comprising a DNA that encodes DGI; a process for preparing DGI comprising culturing the same; a method for identifying a medical compound comprising assaying the action of modulating the DNA gyrase-inhibiting activity of DGI; and a method for identifying a medical compound comprising assaying the action of modulating the expression of a DGI-encoding gene.

摘要翻译： 本发明公开了具有抑制细菌DNA促旋酶活性的能力的蛋白质（DNA促旋酶抑制蛋白质; DGI）; 由包含编码DGI的DNA的可复制表达载体转化的宿主细胞; 制备DGI的方法，包括培养它; 一种用于鉴定医学化合物的方法，包括测定调节DGI的DNA促旋转录酶活性的作用; 以及用于鉴定医学化合物的方法，包括测定调节DGI编码基因表达的作用。