公开(公告)号：US09328366B2

公开(公告)日：2016-05-03

申请号：US13524029

申请日：2012-06-15

Applicant: Sunghoon Kwon ,  Hyoki Kim ,  Howon Lee ,  Sungsik Kim ,  Taehoon Ryu

Inventor： Sunghoon Kwon ,  Hyoki Kim ,  Howon Lee ,  Sungsik Kim ,  Taehoon Ryu

IPC: C12P19/34 ,  B01J19/00 ,  C40B20/02 ,  C40B50/14 ,  C40B60/04

CPC classification number: C12P19/34 ,  B01J2219/00315 ,  B01J2219/00441 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  C40B20/02 ,  C40B50/14 ,  C40B60/04

Abstract: Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale.

Abstract translation: 提供了批量生产高纯度核苷酸的方法，包括提供在固体支持物上具有寡核苷酸克隆文库的测序底物，测序克隆文库，获得测序底物上固体支持物的测量位置数据， 作为使用测量的位置数据进行测序的结果给出的固体支持物产生的信号，使用映射结果从测序底物中提取具有所需碱基序列的固体支持物，并扩增所提取的固体支持物上的寡核苷酸以复制 大规模。

公开(公告)号：US20130109059A1

公开(公告)日：2013-05-02

申请号：US13524029

申请日：2012-06-15

Applicant: Sunghoon KWON ,  Hyoki KIM ,  Howon LEE ,  Sungsik KIM ,  Taehoon RYU

Inventor： Sunghoon KWON ,  Hyoki KIM ,  Howon LEE ,  Sungsik KIM ,  Taehoon RYU

IPC: C12P19/34 ,  B01J19/00

CPC classification number: C12P19/34 ,  B01J2219/00315 ,  B01J2219/00441 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  C40B20/02 ,  C40B50/14 ,  C40B60/04

Abstract: Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale.

Abstract translation: 提供了批量生产高纯度核苷酸的方法，包括提供在固体支持物上具有寡核苷酸克隆文库的测序底物，测序克隆文库，获得测序底物上固体支持物的测量位置数据， 作为使用测量的位置数据进行测序的结果给出的固体支持物产生的信号，使用映射结果从测序底物中提取具有所需碱基序列的固体支持物，并扩增所提取的固体支持物上的寡核苷酸以复制 大规模。