公开(公告)号：US20100276303A1

公开(公告)日：2010-11-04

申请号：US12377497

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Takahiro Takasu ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Takahiro Takasu ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N33/49

CPC分类号： G01N27/3274

摘要： A voltage is applied across a counter electrode and a working electrode while an oxidant of a redox substance is in contact with the counter electrode and is not substantially in contact with the working electrode, and thereby metal that readily undergoes electrolytic oxidation forming at least part of a surface of the working electrode is oxidized while the oxidant in contact with the working electrode is reduced, such that a current produced upon the oxidation and the reduction is measured. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, a hematocrit value of a blood sample can be measured stably with satisfactory detection sensitivity. This measurement can be carried out, for example, with a sensor chip comprising a working electrode 11, a counter electrode 12, and a blood sample holder 14 in communication with a blood sample inlet 16, wherein a portion 31 of the working electrode 11 is disposed closer to the blood sample inlet 16 than a portion 32 of the counter electrode is, a surface of the portion 31 includes the metal that readily undergoes electrolytic oxidation, and a reagent 13 containing the oxidant is disposed in contact with the portion 32.

摘要翻译： 在对电极和工作电极之间施加电压，而氧化还原物质的氧化剂与对电极接触并且基本上不与工作电极接触，从而容易经历电解氧化形成的金属至少部分 工作电极的表面被氧化，而与工作电极接触的氧化剂减少，从而测量氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的血细胞比容值。 该测定例如可以使用包含与血液检体入口16连通的工作电极11，对电极12和血液检体保持器14的传感器芯片进行，其中工作电极11的部分31为 比对置电极的部分32更靠近血液采样入口16设置，部分31的表面包括容易经历电解氧化的金属，并且含有氧化剂的试剂13设置成与部分32接触。