公开(公告)号：US08163506B2

公开(公告)日：2012-04-24

申请号：US10561022

申请日：2004-03-05

申请人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

发明人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

IPC分类号： G01N33/567 ,  C12N15/117 ,  C07K14/705

CPC分类号： G01N33/502 ,  C07K14/705 ,  G01N33/5008 ,  G01N33/5044 ,  Y10S514/885 ,  Y10S530/837 ,  Y10S530/844

摘要： Swine Toll-like receptor 9 (TLR9)-expressing cells are prepared by cloning a TLR9 gene from swine intestinal Peyer's patches. Functional analysis on CpG DNAs using the above cells revealed that swine TLR9 shows a higher recognition ability for a human CpG DNA motif (CpG2006) than for a mouse-specific CpG DNA motif (CpG1826). When the mRNA expression levels in various tissues are compared by the real-time PCR method, it is found out that the mRNA is expressed in Peyer's patches and mesenteric lymph nodes, which play important roles in the intestinal tract immune system, at a level thrice as much as in spleen or more. Thus, the cells expressing an intestinal tract tissue-expressed TLR (for example, TLR9) can be used to identify samples capable of activating the intestinal tract immune system.

摘要翻译： 通过克隆来自猪肠粘膜贴剂的TLR9基因制备表达Toll样受体9（TLR9）的细胞。 使用上述细胞对CpG DNA的功能分析表明，猪TLR9对人CpG DNA基序（CpG2006）的识别能力高于小鼠特异性CpG DNA基序（CpG1826）。 当通过实时PCR方法比较各种组织中的mRNA表达水平时，发现mRNA在肠粘膜和肠系膜淋巴结中表达，其在肠道免疫系统中起重要作用，一级三次 多于脾或更多。 因此，表达肠道组织表达的TLR的细胞（例如，TL​​R9）可用于鉴定能够激活肠道免疫系统的样品。

公开(公告)号：US20220110934A1

公开(公告)日：2022-04-14

申请号：US17645509

申请日：2021-12-22

申请人： Takashi SATO ,  Takeshi Shimosato ,  Tatsuru Moritani ,  Tadahiko Morinaga ,  Yuichi Sato ,  Akihiro Kotsugai

发明人： Takashi SATO ,  Takeshi Shimosato ,  Tatsuru Moritani ,  Tadahiko Morinaga ,  Yuichi Sato ,  Akihiro Kotsugai

IPC分类号： A61K31/496 ,  A61K9/16

摘要： A pharmaceutical composition including particles each containing a water-soluble base material and a poorly water-soluble compound, the water-soluble base material containing a rapidly water-soluble compound, wherein the poorly water-soluble compound is a kinase inhibitor and exists in an amorphous state in the water-soluble base material.

公开(公告)号：US20070298449A1

公开(公告)日：2007-12-27

申请号：US10561022

申请日：2004-03-05

申请人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

发明人： Tadao Saito ,  Takeshi Shimosato ,  Haruki Kitazawa

IPC分类号： C12N15/87 ,  C12N5/06 ,  C12Q1/02

CPC分类号： G01N33/502 ,  C07K14/705 ,  G01N33/5008 ,  G01N33/5044 ,  Y10S514/885 ,  Y10S530/837 ,  Y10S530/844

摘要： Cells with forced expression of swine Toll-like receptor 9 (TLR9) are prepared by cloning a TLR9 gene from swine intestinal Peyer's patches. Functional analysis on CpG DNAs using the above cells revealed that swine TLR9 shows a higher recognition ability for a human CpG DNA motif (CpG2006) than for a mouse-specific CpG DNA motif (CpG1826). When the mRNA expression levels in various tissues are compared by the real-time PCR method, it is found out that the mRNA is expressed in Peyer's patches and mesenteric lymph nodes, which play important roles in the intestinal tract immune system, at a level thrice as much as in spleen or more. Thus, the cells that are forced to express an intestinal tract tissue-expressed TLR (for example, TLR9) can be used to identify samples capable of activating the intestinal tract immune system.

摘要翻译： 通过克隆来自猪肠粘膜斑块的TLR9基因制备猪Toll样受体9（TLR9）强制表达的细胞。 使用上述细胞对CpG DNA的功能分析表明，猪TLR9对人CpG DNA基序（CpG2006）的识别能力高于小鼠特异性CpG DNA基序（CpG1826）。 当通过实时PCR方法比较各种组织中的mRNA表达水平时，发现mRNA在肠粘膜和肠系膜淋巴结中表达，其在肠道免疫系统中起重要作用，一级三次 多于脾或更多。 因此，被迫表达肠道组织表达的TLR（例如，TL​​R9）的细胞可用于鉴定能够激活肠道免疫系统的样品。