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公开(公告)号：US07195892B2
公开(公告)日：2007-03-27
申请号：US10516587
申请日：2003-05-30
申请人： Akiko Nishi , Takumi Mano , Shinichi Yokota , Masayuki Takano , Kazuyoshi Yajima
发明人： Akiko Nishi , Takumi Mano , Shinichi Yokota , Masayuki Takano , Kazuyoshi Yajima
摘要： The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a β-lactam antibiotic such as amoxycillin using said β-lactam acylase. A β-lactam acylase gene of Stenotrophomonas maltophilia was cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a Stenotrophomonas β-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having β-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.
摘要翻译： 本发明的主题是提供具有高活性的β-内酰胺酰基转移酶蛋白，编码所述β-内酰胺酰基转移酶蛋白的基因，具有所述基因的重组载体，含有所述重组载体的转化体，以及制备 β-内酰胺抗生素如阿莫西林，使用所述β-内酰胺酰基转移酶。 克隆了嗜麦芽寡养单胞菌的β-内酰胺酰基转移酶基因，测定了DNA碱基序列和预期的氨基酸序列，得到了嗜麦芽寡养单胞菌β-内酰胺酰基转移酶基因。 发现该基因编码具有约70kDa分子量且具有β-内酰胺酰基转移酶活性的蛋白质，并且可以有效地产生阿莫西林而不被苯乙酸等抑制。此外，通过修饰氨基酸序列，a 可以获得更有效地产生阿莫西林的蛋白质。
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公开(公告)号：US20060035363A1
公开(公告)日：2006-02-16
申请号：US10516587
申请日：2003-05-30
申请人： Akiko Nishi , Takumi Mano , Shinichi Yokota , Masayuki Takano , Kazuyoshi Yajima
发明人： Akiko Nishi , Takumi Mano , Shinichi Yokota , Masayuki Takano , Kazuyoshi Yajima
摘要： The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a β-lactam antibiotic such as amoxycillin using said β-lactam acylase. A β-lactam acylase gene of Stenotrophomonas maltophilia was cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a Stenotrophomonas β-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having β-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.
摘要翻译： 本发明的主题是提供具有高活性的β-内酰胺酰基转移酶蛋白，编码所述β-内酰胺酰基转移酶蛋白的基因，具有所述基因的重组载体，含有所述重组载体的转化体，以及制备 β-内酰胺抗生素如阿莫西林，使用所述β-内酰胺酰基转移酶。 克隆了嗜麦芽寡养单胞菌的β-内酰胺酰基转移酶基因，测定了DNA碱基序列和预期的氨基酸序列，得到了嗜麦芽寡养单胞菌β-内酰胺酰基转移酶基因。 发现该基因编码具有约70kDa分子量且具有β-内酰胺酰基转移酶活性的蛋白质，并且可以有效地产生阿莫西林而不被苯乙酸等抑制。此外，通过修饰氨基酸序列，a 可以获得更有效地产生阿莫西林的蛋白质。
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