公开(公告)号：US07074622B2

公开(公告)日：2006-07-11

申请号：US10988920

申请日：2004-11-15

申请人： Tiecheng A. Qiao ,  Yun C. Chang ,  Eric R. Schmittou ,  Thomas I. Penner

发明人： Tiecheng A. Qiao ,  Yun C. Chang ,  Eric R. Schmittou ,  Thomas I. Penner

IPC分类号： G01N33/543 ,  G01N33/532 ,  G01N33/53 ,  C07C39/04 ,  C09D189/00

CPC分类号： G01N33/544 ,  C12Q1/28 ,  G01N33/569 ,  Y10S436/824 ,  Y10S530/812

摘要： The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle and at least one peroxidase enzyme, contacting a gelatin surface with the suspending solution, adding developer to the suspending solution in contact with the gelatin surface and in the presence of phenol to attach the target particle to the gelatin surface, and washing the gelatin surface to remove unattached particles. The method may also include detecting the presence of the target particle on the gelatin surface as well as detaching and recovering the attached particles after removal of the non-target particles.

摘要翻译： 本发明涉及一种颗粒分离方法，包括在悬浮液中制备含有至少一种可识别目标颗粒的颗粒的悬浮液，用共轭标记物标记目标颗粒，其中该共轭标记包括用于可识别的至少一个识别单元 目标颗粒和至少一种过氧化物酶，使明胶表面与悬浮液接触，将显影剂加入到与明胶表面接触的悬浮液中，并在苯酚存在下将目标颗粒附着到明胶表面，并洗涤明胶 表面去除未附着的颗粒。 该方法还可以包括在明胶表面上检测目标颗粒的存在以及在去除非目标颗粒之后分离和回收附着的颗粒。