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公开(公告)号：US09284659B2
公开(公告)日：2016-03-15
申请号：US13806149
申请日：2011-06-30
申请人： Christian Betzel , Rolf Hilgenfeld , Dierk Hilterhaus , Thomas Klupsch , Peter Muehlig , Karsten Dierks , Arne Meyer
发明人： Christian Betzel , Rolf Hilgenfeld , Dierk Hilterhaus , Thomas Klupsch , Peter Muehlig , Karsten Dierks , Arne Meyer
摘要： A method for crystallizing a substance dissolved in a solvent, including the following steps: introducing a solvent volume containing the substance into a chamber having a preset temperature, humidity, and gas composition, adding a predetermined volume of a precipitant to the solvent volume containing the substance, allowing the solvent to evaporate while simultaneously observing structural changes in the solvent volume containing the substance and the precipitant by means of dynamic light scattering, detecting weight changes and determining the molarities, making an association with the location in the phase diagram on the basis of the DLS measurement and the results of the molarity determination, allowing a predetermined number of crystal nuclei to form by adding solvent or adding precipitant, putting the solvent volume containing the substance and the precipitant into a metastable state by adding solvent and/or protein solution or by allowing the concentration of the dissolved substance to decrease by allowing nucleation cores to form, maintaining the metastable state by adding a predetermined amount of the substance to the solvent volume containing the substance and the precipitant or allowing the solvent to evaporate until at least one crystal of a predetermined size is formed.
摘要翻译： 一种使溶解在溶剂中的物质结晶的方法，包括以下步骤：将含有物质的溶剂体积引入具有预设温度，湿度和气体组成的室中，将预定体积的沉淀剂加入到含有 物质，允许溶剂蒸发，同时通过动态光散射同时观察包含物质和沉淀剂的溶剂体积的结构变化，检测重量变化并确定摩尔数，在相图中与位置相关联 的DLS测量结果和摩尔浓度测定结果，通过加入溶剂或加入沉淀剂形成预定数量的晶核，通过加入溶剂和/或蛋白质溶液将含有物质和沉淀剂的溶剂体积放入亚稳态 或通过允许浓度溶解的子 通过允许成核核心形成，通过向含有物质和沉淀剂的溶剂体积中加入预定量的物质或使溶剂蒸发直到形成至少一种预定尺寸的晶体来保持亚稳态。
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2.发明授权公开(公告)号：US07137734B2
公开(公告)日：2006-11-21
申请号：US10504359
申请日：2003-02-11
IPC分类号： G01N25/02
CPC分类号： G01N25/02 , G01K17/006 , G01N25/4846
摘要： The invention relates to a device for measuring quantities of heat while simultaneously measuring the evaporation kinetics and/or condensation kinetics of the most minute amounts of liquid in order to determine thermodynamic parameters. The aim of the invention is to determine low thermal outputs, which are absorbed or released by the sample, as well as small differences between thermal outputs with regard to a reference measurement of the same magnitude. To this end, a most minute amount of liquid is located inside a measuring chamber having a constant temperature and air humidity. At least one thermal sensor is provided for repeatedly measuring the thermal radiation emitted from the most minute amount of liquid. A measuring means serves to determine the time-dependent change in the most minute amount of liquid. A computer is assigned to the measuring chamber in order to register, display, evaluate and/or subsequently process the measured values.
摘要翻译： 本发明涉及一种用于测量热量的装置，同时测量最少量液体的蒸发动力学和/或冷凝动力学，以便确定热力学参数。 本发明的目的是确定由样品吸收或释放的低热输出以及相对于相同量值的参考测量的热输出之间的小差异。 为此，最小量的液体位于具有恒定温度和空气湿度的测量室内。 提供至少一个热传感器用于重复测量从最微量的液体发射的热辐射。 测量装置用于确定最微量的液体的时间依赖性变化。 计算机被分配到测量室以便注册，显示，评估和/或随后处理测量值。
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公开(公告)号：US20130139749A1
公开(公告)日：2013-06-06
申请号：US13806149
申请日：2011-06-30
申请人： Christian Betzel , Rolf Hilgenfeld , Dierk Hilterhaus , Thomas Klupsch , Peter Muehlig , Karsten Dierks , Arne Meyer
发明人： Christian Betzel , Rolf Hilgenfeld , Dierk Hilterhaus , Thomas Klupsch , Peter Muehlig , Karsten Dierks , Arne Meyer
IPC分类号： C30B7/02
摘要： A method for crystallizing a substance dissolved in a solvent, including the following steps: introducing a solvent volume containing the substance into a chamber having a preset temperature, humidity, and gas composition, adding a predetermined volume of a precipitant to the solvent volume containing the substance, allowing the solvent to evaporate while simultaneously observing structural changes in the solvent volume containing the substance and the precipitant by means of dynamic light scattering, detecting weight changes and determining the molarities, making an association with the location in the phase diagram on the basis of the DLS measurement and the results of the molarity determination, allowing a predetermined number of crystal nuclei to form by adding solvent or adding precipitant, putting the solvent volume containing the substance and the precipitant into a metastable state by adding solvent and/or protein solution or by allowing the concentration of the dissolved substance to decrease by allowing nucleation cores to form, maintaining the metastable state by adding a predetermined amount of the substance to the solvent volume containing the substance and the precipitant or allowing the solvent to evaporate until at least one crystal of a predetermined size is formed.
摘要翻译： 一种使溶解在溶剂中的物质结晶的方法，包括以下步骤：将含有物质的溶剂体积引入具有预设温度，湿度和气体组成的室中，将预定体积的沉淀剂加入到含有 物质，允许溶剂蒸发，同时通过动态光散射同时观察包含物质和沉淀剂的溶剂体积的结构变化，检测重量变化并确定摩尔数，在相图中与位置相关联 的DLS测量结果和摩尔浓度测定结果，通过加入溶剂或加入沉淀剂形成预定数量的晶核，通过加入溶剂和/或蛋白质溶液将含有物质和沉淀剂的溶剂体积放入亚稳态 或通过允许浓度溶解的子 通过允许成核核心形成，通过向含有物质和沉淀剂的溶剂体积中加入预定量的物质或使溶剂蒸发直到形成至少一种预定尺寸的晶体来保持亚稳态。
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4.发明申请公开(公告)号：US20050141587A1
公开(公告)日：2005-06-30
申请号：US10504359
申请日：2003-02-11
申请人： Peter Muhlig , Thomas Klupsch , Rolf Hilgenfeld , Hans-Jurgen Kiel , Axel Walter
发明人： Peter Muhlig , Thomas Klupsch , Rolf Hilgenfeld , Hans-Jurgen Kiel , Axel Walter
CPC分类号： G01N25/02 , G01K17/006 , G01N25/4846
摘要： The invention relates to a device for measuring quantities of heat while simultaneously measuring the evaporation kinetics and/or condensation kinetics of the most minute amounts of liquid in order to determine thermodynamic parameters. The aim of the invention is to determine low thermal outputs, which are absorbed or released by the sample, as well as small differences between thermal outputs with regard to a reference measurement of the same magnitude. To this end, a most minute amount of liquid is located inside a measuring chamber having a constant temperature and air humidity. At least one thermal sensor is provided for repeatedly measuring the thermal radiation emitted from the most minute amount of liquid. A measuring means serves to determine the time-dependent change in the most minute amount of liquid. A computer is assigned to the measuring chamber in order to register, display, evaluate and/or subsequently process the measured values.
摘要翻译： 本发明涉及一种用于测量热量的装置，同时测量最微量的液体的蒸发动力学和/或冷凝动力学，以便确定热力学参数。 本发明的目的是确定由样品吸收或释放的低热输出以及相对于相同量值的参考测量的热输出之间的小差异。 为此，最小量的液体位于具有恒定温度和空气湿度的测量室内。 提供至少一个热传感器用于重复测量从最微量的液体发射的热辐射。 测量装置用于确定最微量的液体的时间依赖性变化。 计算机被分配到测量室以便注册，显示，评估和/或随后处理测量值。
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