公开(公告)号：US09902990B2

公开(公告)日：2018-02-27

申请号：US14122653

申请日：2011-05-27

申请人： Carl Lars Genghis Hansen ,  Michael VanInsberghe ,  Adam White ,  Oleh Petriv ,  Tim Leaver ,  Anupam Singhal ,  William Bowden ,  Veronique Lecault ,  Dan Da Costa ,  Leo Wu ,  Georgia Russell ,  Darek Sikorski

发明人： Carl Lars Genghis Hansen ,  Michael VanInsberghe ,  Adam White ,  Oleh Petriv ,  Tim Leaver ,  Anupam Singhal ,  William Bowden ,  Veronique Lecault ,  Dan Da Costa ,  Leo Wu ,  Georgia Russell ,  Darek Sikorski

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  C12M3/06 ,  B01L3/00 ,  G01N1/34 ,  B01L7/00

CPC分类号： C12Q1/6806 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/086 ,  C12M23/16 ,  C12Q1/6844 ,  G01N1/34 ,  G01N33/5005 ,  G01N33/54366 ,  G01N33/569

摘要： Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.