公开(公告)号：US20060051737A1

公开(公告)日：2006-03-09

申请号：US11203806

申请日：2005-08-15

申请人： Titia Lange ,  Joshua Silverman

发明人： Titia Lange ,  Joshua Silverman

IPC分类号： C12Q1/00 ,  C12Q1/68

CPC分类号： C12Q1/485 ,  G01N2500/10 ,  G01N2800/2835

摘要： The present invention relates to human Rif1 (hRif1) nucleic acid sequences and human Rif1 amino acid sequences encoded therefrom. The invention also encompasses antibodies that are immunologically specific for human Rif1 polypeptides. Also included in the present invention are methods directed to detecting Rif1 foci formed in response to various treatments that induce DNA damage. The formation of foci comprising Rif1 in response to DNA damage serves as a positive indicator of the presence of wild type ATM kinase activity in a cell, whereas the absence of detectable Rif1 foci under such circumstances serves to identify a cell with reduced ATM kinase activity. Modulators of ATM kinase activity and/or Rif1 activity and methods for identifying such modulators are also included in the present invention.

摘要翻译： 本发明涉及人Rif1（hRif1）核酸序列和由其编码的人Rif1氨基酸序列。 本发明还包括对人Rif1多肽具有免疫学特异性的抗体。 本发明还包括用于检测响应诱导DNA损伤的各种治疗形成的Rif1病灶的方法。 包含Rif1响应于DNA损伤的病灶的形成作为细胞中存在野生型ATM激酶活性的阳性指标，而在这种情况下不存在可检测的Rif1病灶用于鉴定具有降低的ATM激酶活性的细胞。 ATM激酶活性和/或Rif1活性的调节剂和鉴定这种调节剂的方法也包括在本发明中。