公开(公告)号：US20100183672A1

公开(公告)日：2010-07-22

申请号：US12376625

申请日：2007-08-06

申请人： Tomomi Kanehara ,  Hiroyuki Yokote ,  Kunio Ohkuma ,  Masahiko Kuranaga ,  Shigeru Morikawa

发明人： Tomomi Kanehara ,  Hiroyuki Yokote ,  Kunio Ohkuma ,  Masahiko Kuranaga ,  Shigeru Morikawa

IPC分类号： A61K39/285 ,  A61P31/20

CPC分类号： A61K39/275 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2710/24134

摘要： A safer live smallpox vaccine, which contains a lowered content of revertants, is provided. A process for manufacturing a live smallpox vaccine which comprises steps of: inoculating a master seed solution of an attenuated vaccinia virus to an appropriate number of containers (1 to n wherein n is an integer) of rabbit kidney cells and incubating them; inoculating a portion of the cultured solution obtained from each container to RK-13 cells and to Vero E6 cells and incubating them to thereby select containers which contain a cultured solution that forms plaques in RK-13 cells but not in Vero E6 cells; and preparing a drug substance of vaccine using the aforementioned cultured solution (working seed solution), and a live smallpox vaccine prepared in the aforementioned process.

摘要翻译： 提供了一种更安全的活的天花疫苗，其中含有较低含量的反转体。 一种活天花疫苗的制造方法，包括以下步骤：将减毒痘苗病毒的母种子溶液接种到适合数量的兔肾细胞的容器（1至n，其中n为整数）并孵育; 将从每个容器获得的培养溶液的一部分接种到RK-13​​细胞和Vero E6细胞并孵育，从而选择含有在RK-13​​细胞中形成斑块但不在Vero E6细胞中的培养溶液的容器; 并使用上述培养液（工作种子溶液）制备疫苗的药物物质，以及在上述方法中制备的活天花疫苗。

公开(公告)号：US08030020B2

公开(公告)日：2011-10-04

申请号：US12376625

申请日：2007-08-06

申请人： Tomomi Kanehara ,  Hiroyuki Yokote ,  Kunio Ohkuma ,  Masahiko Kuranaga ,  Shigeru Morikawa

发明人： Tomomi Kanehara ,  Hiroyuki Yokote ,  Kunio Ohkuma ,  Masahiko Kuranaga ,  Shigeru Morikawa

IPC分类号： C12P1/00 ,  C12N5/00

CPC分类号： A61K39/275 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2710/24134

摘要： A safer live smallpox vaccine, which contains a lowered content of revertants, is provided. A process for manufacturing a live smallpox vaccine which comprises steps of: inoculating a master seed solution of an attenuated vaccinia virus to an appropriate number of containers (1 to n wherein n is an integer) of rabbit kidney cells and incubating them; inoculating a portion of the cultured solution obtained from each container to RK-13 cells and to Vero E6 cells and incubating them to thereby select containers which contain a cultured solution that forms plaques in RK-13 cells but not in Vero E6 cells; and preparing a drug substance of vaccine using the aforementioned cultured solution (working seed solution), and a live smallpox vaccine prepared in the aforementioned process.

摘要翻译： 提供了一种更安全的活的天花疫苗，其中含有较低含量的反转体。 一种活天花疫苗的制造方法，包括以下步骤：将减毒痘苗病毒的母种子溶液接种到适合数量的兔肾细胞的容器（1至n，其中n为整数）并孵育; 将从每个容器获得的培养溶液的一部分接种到RK-13​​细胞和Vero E6细胞并孵育，从而选择含有在RK-13​​细胞中形成斑块但不在Vero E6细胞中的培养溶液的容器; 并使用上述培养液（工作种子溶液）制备疫苗的药物物质，以及在上述方法中制备的活天花疫苗。