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    ASSAY METHODS FOR MDV-1
    1.
    发明申请
    ASSAY METHODS FOR MDV-1 有权
    MDV-1的测定方法

    公开(公告)号：US20120208177A1

    公开(公告)日：2012-08-16

    申请号：US13391563

    申请日：2010-08-25

    申请人： Susan Baigent ,  Venugopal Nair ,  Hervé Le Galludec

    发明人： Susan Baigent ,  Venugopal Nair ,  Hervé Le Galludec

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/705 ,  C12Q2600/156

    摘要： A method for the quantification of a vaccine strain and/or a virulent strain of Wlarek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp3B gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic aα′d probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses.

    摘要翻译： 一种用于定量来自鸟的样品中的Wlarek's病毒血清型-1（MDV-1）的疫苗株和/或毒力株的方法，包括以下步骤：（i）从鸟提供生物样品， 任选地从生物样品中分离核酸; （ii）对（i）生物样品进行实时定量PCR（qPCR），包括：（a）在（i）的核酸样品中扩增pp3B基因的区域，所述区域含有一致的单核苷酸 疫苗与MDV-1毒株之间的多态性（SNP）差异; 和（b）使（a）的扩增的核酸与对MDV-1的疫苗株的SNP特异性的可检测的核酸探针和/或对于毒性毒株的SNP特异性的可检测的核酸探针接触 MDV-1; 和（iii）测量由（ii）的探针产生的可检测信号的变化。 还提供了用于绝对定量疫苗和毒性病毒的方法。

    Assay methods for MDV-1
    3.
    发明授权
    Assay methods for MDV-1 有权
    MDV-1的测定方法

    公开(公告)号：US08865409B2

    公开(公告)日：2014-10-21

    申请号：US13391563

    申请日：2010-08-25

    申请人： Susan Baigent ,  Venugopal Nair ,  Hervé Le Galludec

    发明人： Susan Baigent ,  Venugopal Nair ,  Hervé Le Galludec

    IPC分类号： C12Q1/70 ,  C12N15/11

    CPC分类号： C12Q1/705 ,  C12Q2600/156

    摘要： A method for the quantification of a vaccine strain and/or a virulent strain of Marek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp38 gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic acid probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses.

    摘要翻译： 一种用于在来自鸟的样品中定量疫苗株和/或马立克氏病病毒血清型1（MDV-1）的毒性菌株的方法，包括以下步骤：（i）从鸟提供生物样品， 任选地从生物样品中分离核酸; （ii）对（i）的生物样品进行实时定量PCR（qPCR），包括：（a）在（i）的核酸样品中扩增pp38基因的区域，所述区域含有一致的单核苷酸 疫苗与MDV-1毒株之间的多态性（SNP）差异; 和（b）使（a）的扩增的核酸与对MDV-1的疫苗株的SNP特异的可检测的核酸探针和/或特异于MDV-1的毒力株的SNP的可检测的核酸探针接触， 1; 和（iii）测量由（ii）的探针产生的可检测信号的变化。 还提供了用于绝对定量疫苗和毒性病毒的方法。