公开(公告)号：US07306917B2

公开(公告)日：2007-12-11

申请号：US11222565

申请日：2005-09-09

申请人： James R. Prudent ,  Jeff G. Hall ,  Victor L. Lyamichev ,  Mary Ann D. Brow ,  James E. Dahlberg

发明人： James R. Prudent ,  Jeff G. Hall ,  Victor L. Lyamichev ,  Mary Ann D. Brow ,  James E. Dahlberg

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N9/22 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/683 ,  Y10S435/81 ,  Y10S435/822 ,  C12Q2565/525 ,  C12Q2561/109 ,  C12Q2525/301 ,  C12Q2563/149

摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。