公开(公告)号：US20240352521A1

公开(公告)日：2024-10-24

申请号：US18387669

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Luigi Jhon ALVARADO MARTINEZ

IPC: C12Q1/6876 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874

CPC classification number: C12Q1/6876 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6874 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20230272458A1

公开(公告)日：2023-08-31

申请号：US17990565

申请日：2022-11-18

Applicant: Sherlock Biosciences, Inc.

Inventor： William Jeremy Blake ,  Xiang Li ,  Mary Katherine Wilson ,  Christine Marie Coticchia ,  Brendan John Manning ,  Pradeep Ramesh

IPC: C12Q1/683 ,  C12N15/113 ,  C12N9/22 ,  C12Q1/6844

CPC classification number: C12Q1/683 ,  C12N15/113 ,  C12N9/22 ,  C12Q1/6844 ,  C12N2320/10 ,  C12N2310/20

Abstract: The present disclosure provides improved detection (e.g., diagnostic) assays that utilize a Cas protein collateral cleavage activity.

公开(公告)号：US11732302B2

公开(公告)日：2023-08-22

申请号：US17831356

申请日：2022-06-02

Applicant: 10X GENOMICS, INC.

Inventor： Phillip Belgrader ,  Josephine Harada ,  Tarjei Sigurd Mikkelsen ,  Katherine Pfeiffer ,  Serge Saxonov ,  John R. Stuelpnagel

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12N15/1075 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20230203577A1

公开(公告)日：2023-06-29

申请号：US17822117

申请日：2022-08-24

Applicant: 10x Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2525/191

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20230203568A1

公开(公告)日：2023-06-29

申请号：US17792638

申请日：2021-01-14

Applicant: The Broad Institute, Inc. ,  Dana-Farber Cancer Institute, Inc.

Inventor： Viktor A. Adalsteinsson ,  Gregory Gydush ,  Gerassimos Makrigiorgos ,  Erica Nguyen

IPC: C12Q1/683 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q1/6869

CPC classification number: C12Q1/683 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/156

Abstract: The disclosure provides novel methods, compositions, and kits that combine hybrid capture using short allele-specific probes with duplex molecular” barcoding and noise modeling within each sample to afford high accuracy sequencing of rare mutations at low cost.

公开(公告)号：US20190194755A1

公开(公告)日：2019-06-27

申请号：US16324576

申请日：2017-08-10

Applicant: Ramot at Tel-Aviv University Ltd. ,  Rheinisch-Westfalische Technische Hochschule (RWTH) Aachen

Inventor： Yuval EBENSTEIN ,  Elmar WEINHOLD ,  Assaf GRUNWALD ,  Tslil GABRIELI

IPC: C12Q1/6883 ,  C12Q1/683

CPC classification number: C12Q1/6883 ,  C12Q1/683 ,  C12Q2600/154 ,  C12Y301/21004

Abstract: A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises: (a) determining the number of repeats of a DNA sequence in DNA molecules of a sample of the subject; and (b) determining the CpG methylation status of the DNA molecules, wherein the number of repeats of the DNA sequence and the CpG methylation status of the DNA molecules is indicative of the disease.

公开(公告)号：US20190032129A1

公开(公告)日：2019-01-31

申请号：US16144832

申请日：2018-09-27

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov ,  Rajiv Bharadwaj

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190024166A1

公开(公告)日：2019-01-24

申请号：US16138448

申请日：2018-09-21

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6804 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US09970898B2

公开(公告)日：2018-05-15

申请号：US14771989

申请日：2014-03-11

Applicant: The University of North Carolina at Chapel Hill

Inventor： John Michael Ramsey ,  Laurent Menard

IPC: C12M1/00 ,  G01N27/447 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2300/0896 ,  B01L2400/0418 ,  B01L2400/0421 ,  C12Q1/683 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2561/113 ,  C12Q2565/631

Abstract: Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.

公开(公告)号：US20180016631A1

公开(公告)日：2018-01-18

申请号：US15539273

申请日：2015-12-23

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/10 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/683 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2525/131 ,  C12Q2525/155 ,  C12Q2535/122

Abstract: Disclosed is a method suitable for (long-range) mate pair sequencing wherein the mate pairs are located within a certain distance from each other on the same nucleotide sequence. By ligating a DNA fragment into an identifier section—containing backbone, a digestable circularized construct is provided to which adaptors can be ligated after digestion. Amplification yields amplicons that contain a combination of the identifier section with the terminal part of the fragments. The fragments are subsequently mated to each other to obtain a mated pair by identifying the corresponding identifier section in both amplicons. The mated pairs can be used in the construction of genome scaffolds or in the generation of draft genome sequences.