公开(公告)号：US20120257203A1

公开(公告)日：2012-10-11

申请号：US13444696

申请日：2012-04-11

申请人： Enrico Gratton ,  Alexander Dvornikov ,  Viera Crosignani

发明人： Enrico Gratton ,  Alexander Dvornikov ,  Viera Crosignani

IPC分类号： G01N21/00

CPC分类号： G01N21/6456 ,  G01N21/4795

摘要： An apparatus and method for in-depth fluorescence imaging using two-photon fluorescence imaging in turbid media. The apparatus includes a detector which can significantly enhance the use of a detection method that allows to efficiently collect scattered fluorescence photons from a wide area of the turbid sample. By using this detector it is possible to perform imaging of turbid samples, simulating brain tissue at depths up to 3 mm, where the two-photon induced fluorescence signal is too weak to be detected by previous means used in conventional two-photon microscopy. The detector separates the excitation and detection optics which allows a more efficient collection of fluorescence and enhancing the possible imaging depth.

摘要翻译： 用于在混浊介质中使用双光子荧光成像进行深度荧光成像的装置和方法。 该装置包括能够显着增强检测方法的使用的检测器，该检测方法允许有效地从浑浊样品的广泛区域收集散射的荧光光子。 通过使用该检测器，可以进行浑浊样品的成像，模拟高达3mm深度的脑组织，其中双光子诱导的荧光信号太弱，无法通过传统双光子显微镜中使用的先前手段检测。 检测器分离激发和检测光学器件，其允许更有效地收集荧光并增强可能的成像深度。

公开(公告)号：US08692998B2

公开(公告)日：2014-04-08

申请号：US13444696

申请日：2012-04-11

申请人： Enrico Gratton ,  Alexander Dvornikov ,  Viera Crosignani

发明人： Enrico Gratton ,  Alexander Dvornikov ,  Viera Crosignani

IPC分类号： G01N21/00

CPC分类号： G01N21/6456 ,  G01N21/4795

摘要： An apparatus and method for in-depth fluorescence imaging using two-photon fluorescence imaging in turbid media. The apparatus includes a detector which can significantly enhance the use of a detection method that allows to efficiently collect scattered fluorescence photons from a wide area of the turbid sample. By using this detector it is possible to perform imaging of turbid samples, simulating brain tissue at depths up to 3 mm, where the two-photon induced fluorescence signal is too weak to be detected by previous means used in conventional two-photon microscopy. The detector separates the excitation and detection optics which allows a more efficient collection of fluorescence and enhancing the possible imaging depth.

摘要翻译： 用于在混浊介质中使用双光子荧光成像进行深度荧光成像的装置和方法。 该装置包括能够显着增强检测方法的使用的检测器，该检测方法允许有效地从浑浊样品的广泛区域收集散射的荧光光子。 通过使用该检测器，可以进行浑浊样品的成像，模拟高达3mm深度的脑组织，其中双光子诱导的荧光信号太弱，无法通过传统双光子显微镜中使用的先前手段检测。 检测器分离激发和检测光学器件，其允许更有效地收集荧光并增强可能的成像深度。