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公开(公告)号：US5877004A
公开(公告)日：1999-03-02
申请号：US462334
申请日：1995-06-05
CPC分类号： C07K14/005 , C12N9/2402 , C12N9/2434 , C12Q1/34 , C12Q1/68 , C12Q1/6897 , C12Y302/01031 , C12N2710/16122 , G01N2333/045
摘要： This invention relates to a method for identifying non-essential genes of the human cytomegalovirus (HCMV) genome through the insertion of a .beta.-glucuronidase marker gene into a specified HCMV gene, such that, if the product of the HCMV gene is not expressed, the gene is identified as non-essential for replication of HCMV. This invention also relates to a method of screening for compounds which inhibit HCMV by the insertion of the .beta.-glucuronidase marker gene into a HCMV gene, such that the enzyme marker is expressed and cleaves a conjugate chemical substrate in an assay system to yield a detectable fluorescing product or to result in a color change. This invention further provides the gene responsible in HCMV early cytopathic effect.
摘要翻译： 本发明涉及通过将β-葡糖醛酸糖苷酶标记基因插入特定HCMV基因中鉴定人巨细胞病毒（HCMV）基因组的非必需基因的方法，使得如果HCMV基因的产物未被表达， 该基因被鉴定为对于HCMV的复制非必需的。 本发明还涉及通过将β-葡糖醛酸糖苷酶标记基因插入到HCMV基因中筛选抑制HCMV的化合物的方法，使得酶标记物在测定系统中表达并切割缀合物化学底物以产生可检测的 荧光产品或导致颜色变化。 本发明还提供了负责HCMV早期致细胞病变作用的基因。
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2.发明授权Methods for identifying non-essential genes of the human cytomegalovirus genome and for screening for inhibitors of human cytomegalovirus 失效用于鉴定人巨细胞病毒基因组的非必需基因并筛选人巨细胞病毒抑制剂的方法
公开(公告)号：US5843637A
公开(公告)日：1998-12-01
申请号：US463175
申请日：1995-06-05
CPC分类号： C07K14/005 , C12N9/2402 , C12N9/2434 , C12Q1/34 , C12Q1/68 , C12Q1/6897 , C12Y302/01031 , C12N2710/16122 , G01N2333/045
摘要： This invention relates to a method for identifying non-essential genes of the human cytomegalovirus (HCMV) genome through the insertion of a .beta.-glucuronidase marker gene into a specified HCMV gene, such that, if the product of the HCMV gene is not expressed, the gene is identified as non-essential for replication of HCMV. This invention also relates to a method of screening for compounds which inhibit HCMV by the insertion of the .beta.-glucuronidase marker gene into a HCMV gene, such that the enzyme marker is expressed and cleaves a conjugate chemical substrate in an assay system to yield a detectable fluorescing product or to result in a color change. This invention further provides the gene responsible in HCMV early cytopathic effect.
摘要翻译： 本发明涉及通过将β-葡糖醛酸糖苷酶标记基因插入特定HCMV基因中鉴定人巨细胞病毒（HCMV）基因组的非必需基因的方法，使得如果HCMV基因的产物未被表达， 该基因被鉴定为对于HCMV的复制非必需的。 本发明还涉及通过将β-葡糖醛酸糖苷酶标记基因插入到HCMV基因中筛选抑制HCMV的化合物的方法，使得酶标记物在测定系统中表达并切割缀合物化学底物以产生可检测的 荧光产品或导致颜色变化。 本发明还提供了负责HCMV早期致细胞病变作用的基因。
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