公开(公告)号：US07033758B2

公开(公告)日：2006-04-25

申请号：US09872493

申请日：2001-06-01

申请人： Daryn Kenny ,  Lu Ping Shen ,  Vincent P. Antao ,  Audrey N. Player ,  Wei Cao

发明人： Daryn Kenny ,  Lu Ping Shen ,  Vincent P. Antao ,  Audrey N. Player ,  Wei Cao

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/682 ,  C12Q1/6841 ,  C12Q2523/313

摘要： Methods are provided for highly sensitive and rapid in situ detection of a nucleic acid analyte of a known sequence. The method employs oligonucleotide probes in a series of optimized steps to amplify a signal and decrease background. Sensitivity is enhanced such that the method can detect as few as 1–2 copies of nucleic acid analyte per sample, the sample containing a cell, tissue or similar biological material. Methods of detecting and identifying the position of the nucleic acid analyte in a cell are also provided.