公开(公告)号：US12123874B2

公开(公告)日：2024-10-22

申请号：US16318793

申请日：2017-07-18

Applicant: CELL IDX, INC. ,  THE UNIVERSITY OF CHICAGO

Inventor： David A. Schwartz ,  Stephen J. Kron

IPC: G01N33/547 ,  C12N9/04 ,  C12N9/08 ,  C12N9/16 ,  C12Q1/6804 ,  C12Q1/682 ,  G01N33/533 ,  G01N33/535 ,  G01N33/543

CPC classification number: G01N33/547 ,  C12N9/0006 ,  C12N9/0065 ,  C12N9/16 ,  C12Q1/6804 ,  C12Q1/682 ,  G01N33/533 ,  G01N33/535 ,  G01N33/54353 ,  C12Y101/03004 ,  C12Y111/01007 ,  C12Y301/03001 ,  C12Q1/682 ,  C12Q2531/101 ,  C12Q2531/125 ,  C12Q2543/101 ,  C12Q1/6804 ,  C12Q2531/101 ,  C12Q2531/125 ,  C12Q2543/101

Abstract: The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids. Also provided are detectable antibodies specific for the bridging antigens, kits comprising the reagent compounds and antibody and oligonucleotide reagents, methods of signal amplification using the compounds and reagents of the disclosure, methods of preparation of the compounds and reagents, and compositions comprising the compounds and reagents.

公开(公告)号：US20240263230A1

公开(公告)日：2024-08-08

申请号：US18592843

申请日：2024-03-01

Applicant: Complete Genomics, Inc

Inventor： Radoje Drmanac ,  Matthew J. Callow ,  Snezana Drmanac ,  Brian K. Hauser ,  George Yeung

IPC: C12Q1/6874 ,  C07H21/04 ,  C07K1/04 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434

CPC classification number: C12Q1/6874 ,  C07H21/04 ,  C07K1/047 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N15/1434 ,  C12Q2525/151 ,  C12Q2525/313 ,  C12Q2531/125 ,  C12Q2565/513 ,  Y10S977/778 ,  Y10S977/789 ,  Y10S977/792 ,  Y10S977/88 ,  Y10S977/882

Abstract: Random arrays of single molecules are provided for carrying out large scale analyzes, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 μm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.

公开(公告)号：US20240263220A1

公开(公告)日：2024-08-08

申请号：US18431779

申请日：2024-02-02

Applicant: 10x Genomics, Inc.

Inventor： Mimmi OLOFSSON

IPC: C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827

CPC classification number: C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present disclosure relates in some aspects to methods for analyzing target nucleic acids in a biological sample. In some aspects, the presence, amount, and/or identity of a plurality of different target nucleic acids and/or variant sequences (e.g., SNPs or mutations) of one or more of the different target nucleic acids are analyzed in situ in a sample. Also provided are oligonucleotides, sets of oligonucleotides, compositions, and kits for use in accordance with the methods.

公开(公告)号：US20240229130A9

公开(公告)日：2024-07-11

申请号：US18381400

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

公开(公告)号：US20240229118A1

公开(公告)日：2024-07-11

申请号：US18537266

申请日：2023-12-12

Applicant: Singular Genomics Systems, Inc.

Inventor： Richard Que ,  Martin Maria Fabani ,  Zhenmin Hong ,  Michael Lawson

IPC: C12Q1/6841 ,  C12Q1/682

CPC classification number: C12Q1/6841 ,  C12Q1/682

Abstract: Disclosed herein, inter alia, are methods and compositions for improved circular polynucleotide amplification and detection.

公开(公告)号：US20240229107A1

公开(公告)日：2024-07-11

申请号：US18390804

申请日：2023-12-20

Applicant: Singular Genomics Systems, Inc.

Inventor： Michael Lawson

IPC: C12Q1/682 ,  C12Q1/6876

CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/16

Abstract: Disclosed herein, inter alia, are oligonucleotides, methods, and kits useful for amplifying and detecting targets such as nucleic acids, proteins, and carbohydrates.

公开(公告)号：US20240167082A1

公开(公告)日：2024-05-23

申请号：US18550598

申请日：2022-03-18

Applicant: PARIS SCIENCES ET LETTRES ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS

Inventor： Yannick RONDELEZ ,  Andrew GRIFFITHS ,  Gaël BLIVET-BAILLY ,  Pablo IBANEZ ,  Satyam BANERJEE ,  Guillaume GINES ,  Antoine MASURIER

IPC: C12Q1/682 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/6869

CPC classification number: C12Q1/682 ,  C12N9/1241 ,  C12N15/1075 ,  C12N15/1096 ,  C12Q1/6869 ,  C12Q2600/158

Abstract: The present invention relates to a method for the selective analysis of a subpopulation of cells or organelles based determining the level of at least one cell or organelle marker, via the implementation of an adapted DNA toolbox able to convert the presence of a cell or organelle marker into a signal. This method is particularly useful for selecting cells or organelles having a phenotype of interest, analyzing the proteins of said cells or organelles and/or sequencing the DNA and/or RNA of said cells. The present invention also relates to a kit for implementing the method of selective analysis of cells or organelles as defined above.

公开(公告)号：US11987839B2

公开(公告)日：2024-05-21

申请号：US18372098

申请日：2023-09-24

Applicant: VedaBio, Inc. ,  The Board of Trustees of the University of Illinois

Inventor： Anurup Ganguli ,  Ariana Mostafa ,  Jacob Berger ,  Ashish Pandey

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/682 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2320/10

Abstract: The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.

公开(公告)号：US11965205B1

公开(公告)日：2024-04-23

申请号：US18377294

申请日：2023-10-05

Applicant: VedaBio, Inc.

Inventor： Swetha Murali ,  Anurup Ganguli ,  Ketki Sawant ,  Ravi Vijayendran

IPC: C12Q1/682 ,  C12N9/22

CPC classification number: C12Q1/682 ,  C12N9/22 ,  C12N2310/20

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.

公开(公告)号：US20240124919A1

公开(公告)日：2024-04-18

申请号：US18377294

申请日：2023-10-05

Applicant: VedaBio, Inc.

Inventor： Swetha Murali ,  Anurup Ganguli ,  Ketki Sawant ,  Ravi Vijayendran

IPC: C12Q1/682 ,  C12N9/22

CPC classification number: C12Q1/682 ,  C12N9/22 ,  C12N2310/20

Abstract: The present disclosure relates to compositions of matter and assay methods used to detect one or more non-nucleic acid targets of interest in a sample. The compositions and methods provide signal boost upon detection of non-nucleic acid targets of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 25° C. or less, allow for massive multiplexing, high accuracy, minimal non-specific signal generation, and are easily reprogrammable.