公开(公告)号：US5373053A

公开(公告)日：1994-12-13

申请号：US990584

申请日：1992-12-14

申请人： Rolf H. Berg ,  Kristoffer Almdal ,  Walther B. Pedersen ,  Arne Holm ,  James P. Tam ,  Robert B. Merrifield

发明人： Rolf H. Berg ,  Kristoffer Almdal ,  Walther B. Pedersen ,  Arne Holm ,  James P. Tam ,  Robert B. Merrifield

IPC分类号： C07K1/04 ,  G01N33/545 ,  C07C103/52 ,  C07D51/52

CPC分类号： G01N33/545 ,  C07K1/042

摘要： A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion.Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.

摘要翻译： 以高产率和高纯度固相合成肽或蛋白质的方法使用由官能化聚苯乙烯接枝的聚合物基质组成的固体支持物，所述接枝聚苯乙烯链基本上是非交联并具有链分子量， 不包括任选的非反应性取代基，为至少20万，优选在600,000-1200,000的范围内。 特别合适的聚合物基材是聚烯烃如聚乙烯的基材。 该方法特别适合于以平行和基本上同时的方式区分多种肽或蛋白质的合成。 用于进行合成的固体支持物的优选实施方案由在自由基引发的方法中已经用聚苯乙烯链接枝的薄聚乙烯片或薄膜制备，其中聚乙烯片或薄膜浸渍在任选取代的苯乙烯单体的溶液中 醇如甲醇，溶液中苯乙烯的体积百分比优选为约30％v / v，并进行γ照射。

公开(公告)号：US5258454A

公开(公告)日：1993-11-02

申请号：US882059

申请日：1992-05-12

申请人： Rolf H. Berg ,  Kristoffer Almdal ,  Walther B. Pedersen ,  Arne Holm ,  James P. Tam ,  Robert B. Merrifield

发明人： Rolf H. Berg ,  Kristoffer Almdal ,  Walther B. Pedersen ,  Arne Holm ,  James P. Tam ,  Robert B. Merrifield

IPC分类号： C07K1/04 ,  G01N33/545 ,  C08F283/00 ,  C08G63/48 ,  C08G63/91

CPC分类号： G01N33/545 ,  C07K1/042

摘要： A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid-support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion.Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.

摘要翻译： 用于以高产率和高纯度固相合成肽或蛋白质的方法使用由官能化聚苯乙烯接枝的聚合物基质组成的固体支持物，所述接枝聚苯乙烯链基本上是非交联的且具有链分子量 ，不包括任选的非反应性取代基，为至少20万，优选在600,000-1200,000的范围内。 特别合适的聚合物基材是聚烯烃如聚乙烯的基材。 该方法特别适合于以平行和基本上同时的方式区分多种肽或蛋白质的合成。 用于进行合成的固体支持物的优选实施方案由在自由基引发的方法中已经用聚苯乙烯链接枝的薄聚乙烯片或薄膜制备，其中聚乙烯片或薄膜浸渍在任选取代的苯乙烯单体的溶液中 醇如甲醇，溶液中苯乙烯的体积百分比优选为约30％v / v，并进行γ照射。