公开(公告)号：US06613959B1

公开(公告)日：2003-09-02

申请号：US09371338

申请日：1999-08-10

申请人： Jen Sheen ,  Yelena V. Kovtun ,  Wan-Ling Chiu

发明人： Jen Sheen ,  Yelena V. Kovtun ,  Wan-Ling Chiu

IPC分类号： C12N1509

CPC分类号： C12N9/1205 ,  C12N9/16 ,  C12N15/8266 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8287 ,  C12N15/8294

摘要： The invention features a method for increasing stress resistance or tolerance in a plant, the method including the steps of: (a) introducing into plant cells a transgene including DNA encoding a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) operably linked to a promoter functional in plant cells to yield transformed plant cells; and (b) regenerating a transgenic plant from the transformed cells, wherein the kinase domain of said MAPKKK is expressed in the cells of the transgenic plant, thereby increasing the level of stress resistance or tolerance in the transgenic plant. The invention further features plants including a recombinant transgene capable of expressing a kinase domain of a mitogen-activated protein kinase kinase kinase (MAPKKK) or a kinase domain thereof, wherein the transgene is expressed in said plant under the control of a promoter that is functional in a plant cell.

摘要翻译： 本发明的特征在于增加植物的胁迫抗性或耐受性的方法，所述方法包括以下步骤：（a）可操作地向植物细胞中引入包含编码丝裂原活化蛋白激酶激酶激酶（MAPKKK）的激酶结构域的DNA的转基因 与在植物细胞中起作用的启动子连接以产生转化的植物细胞; 和（b）从转化的细胞再生转基因植物，其中所述MAPKKK的激酶结构域在转基因植物的细胞中表达，由此增加转基因植物的胁迫抗性或耐受性水平。 本发明还涉及包括能够表达丝裂原活化蛋白激酶激酶激酶（MAPKKK）或其激酶结构域的激酶结构域的重组转基因的植物，其中所述植物在所述植物中表达，所述启动子是功能性的 在植物细胞中。

公开(公告)号：US06548743B1

公开(公告)日：2003-04-15

申请号：US09371671

申请日：1999-08-10

申请人： Jen Sheen ,  Wan-Ling Chiu

发明人： Jen Sheen ,  Wan-Ling Chiu

IPC分类号： A01H500

CPC分类号： C12N9/1205 ,  C12N9/16 ,  C12N15/8266 ,  C12N15/827 ,  C12N15/8294

摘要： Disclosed are transgenic plants which include a transgene which expresses a phosphatase domain of a dual-specificity mitogen-activated protein kinase (MAPK) phosphatase, wherein the transgene is expressed in the transgenic plant under the control of a promoter that is functional in a plant cell. Also disclosed are methods which are useful for modifying the phenotype of a plant, including the steps of: (a) introducing into a plant cell a transgene comprising DNA encoding a phosphatase domain of a dual-specificity mitogen-activating protein kinase (MAPK) phosphatase operably linked to a promoter functional in plant cells to yield a transformed plant cell; and (b) regenerating a transgenic plant from the transformed cells, wherein the phosphatase domain of the dual-specificity MAPK phosphatase is expressed in the cells of the transgenic plant, thereby modifying the phenotype of the transgenic plant.

摘要翻译： 披露的是转基因植物，其包括表达双特异性丝裂原活化蛋白激酶（MAPK）磷酸酶的磷酸酶结构域的转基因，其中转基因在转基因植物中表达，其在植物细胞中起作用的启动子 。 还公开了可用于修饰植物表型的方法，包括以下步骤：（a）将包含编码双特异性丝裂原活化蛋白激酶（MAPK）磷酸酶的磷酸酶结构域的DNA的转基因导入植物细胞 可操作地连接到在植物细胞中起作用的启动子以产生转化的植物细胞; 和（b）从转化的细胞再生转基因植物，其中双特异性MAPK磷酸酶的磷酸酶结构域在转基因植物的细胞中表达，由此修饰转基因植物的表型。