公开(公告)号：US10907162B2

公开(公告)日：2021-02-02

申请号：US16791101

申请日：2020-02-14

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/11 ,  C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US10655132B1

公开(公告)日：2020-05-19

申请号：US16174764

申请日：2018-10-30

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a novel SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each structurally-similar molecule in said family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US20200172907A1

公开(公告)日：2020-06-04

申请号：US16791101

申请日：2020-02-14

申请人： Weijuan YANG ,  Haixiang YU ,  Yingzhu LIU ,  Yi XIAO

发明人： Weijuan YANG ,  Haixiang YU ,  Yingzhu LIU ,  Yi XIAO

IPC分类号： C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a novel SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each structurally-similar molecule in said family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US11060095B2

公开(公告)日：2021-07-13

申请号：US17117317

申请日：2020-12-10

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/11 ,  C12N15/115 ,  A61K31/167 ,  A61K31/137 ,  G01N27/26

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US20210102210A1

公开(公告)日：2021-04-08

申请号：US17117317

申请日：2020-12-10

申请人： Weijuan YANG ,  Haixiang YU ,  Yingzhu LIU ,  Yi XIAO

发明人： Weijuan YANG ,  Haixiang YU ,  Yingzhu LIU ,  Yi XIAO

IPC分类号： C12N15/115 ,  G01N27/26 ,  A61K31/137 ,  A61K31/167

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.