公开(公告)号：US20100062455A1

公开(公告)日：2010-03-11

申请号：US12516297

申请日：2007-11-26

申请人： Weng Keong Loke ,  Yong Teng Tan ,  Josefina Seow

发明人： Weng Keong Loke ,  Yong Teng Tan ,  Josefina Seow

IPC分类号： G01N33/53

CPC分类号： C12Q1/46

摘要： The present invention relates to a rapid, high-throughput detection method for screening of potential casualties exposed to a cholinesterase inhibitor. A kit is provided for conducting the method that requires only a small volume of sample such as blood for detection and has a rapid screening throughput for multiple casualties. The kit is able to detect both free and protein-bound-cholinesterase inhibitors. The method should be able to differentiate asymptomatic exposure from the mass psychogenic illness cases.

摘要翻译： 本发明涉及用于筛选暴露于胆碱酯酶抑制剂的潜在伤员的快速，高通量检测方法。 提供了用于进行仅需要少量样品（例如血液）以进行检测的方法的试剂盒，并且具有用于多次伤亡的快速筛选产量。 该试剂盒能够检测游离蛋白和蛋白质结合胆碱酯酶抑制剂。 该方法应能够区分无症状的暴露与大量心理疾病病例。

公开(公告)号：US08187832B2

公开(公告)日：2012-05-29

申请号：US12516297

申请日：2007-11-26

申请人： Weng Keong Loke ,  Yong Teng Tan ,  Josefina Seow

发明人： Weng Keong Loke ,  Yong Teng Tan ,  Josefina Seow

IPC分类号： C12Q1/46

CPC分类号： C12Q1/46

摘要： A method of detecting an amount of a cholinesterase inhibitor in a sample comprising steps of: (a) contacting the sample with an agent capable of recovering the cholinesterase inhibitor from the sample so that the cholinesterase inhibitor is recovered, wherein the agent comprises a reactivity towards phosphyl moieties; (b) isolating the recovered cholinesterase inhibitor from the sample; (c) contacting the isolated cholinesterase inhibitor from step (b) with a test cholinesterase wherein the cholinesterase activity of the test cholinesterase before step (a) is known; and (d) measuring the cholinesterase activity to determine the amount of cholinesterase inhibitor in the sample based on the inhibition of the test cholinesterase activity from the known activity of the test cholinesterase before step (a).

摘要翻译： 一种检测样品中胆碱酯酶抑制剂的量的方法，包括以下步骤：（a）使样品与能够从样品中回收胆碱酯酶抑制剂的试剂接触，从而回收胆碱酯酶抑制剂，其中该试剂包含对 膦基部分; （b）从样品中分离回收的胆碱酯酶抑制剂; （c）将步骤（b）中分离的胆碱酯酶抑制剂与测试胆碱酯酶接触，其中在步骤（a）之前测试胆碱酯酶的胆碱酯酶活性是已知的; 基于在步骤（a）之前从测试胆碱酯酶的已知活性抑制测试胆碱酯酶活性，测量胆碱酯酶活性以确定样品中胆碱酯酶抑制剂的量。

公开(公告)号：US5882850A

公开(公告)日：1999-03-16

申请号：US949617

申请日：1997-10-14

申请人： Eugene Khor ,  Aileen Wee ,  Weng Keong Loke ,  Bee Li Tan

发明人： Eugene Khor ,  Aileen Wee ,  Weng Keong Loke ,  Bee Li Tan

IPC分类号： A01N1/02 ,  A61K31/10 ,  A61L27/36 ,  A61L27/50 ,  A61L27/60

CPC分类号： A61L27/3604 ,  A61L27/3625 ,  A61L27/3687 ,  A61L27/507 ,  A61L27/60 ,  A61L2400/02 ,  Y10S623/916

摘要： Biological materials are treated with an organic chemical agent, dimethyl sulfoxide, to delay the onset of in vivo calcification. The biological materials so treated become resistant to calcification over a prolonged period with no known deleterious effects to the host. The procedure involves the incubation of the natural biological materials in the chemical agent, prior to or after glutaraldehyde fixation, at room temperature or at temperatures up to 60.degree. C. Materials that can be treated with this process include biological materials intended for bioprostheses use, such as porcine aortic valves, porcine aortic valve leaflets, bovine, equine and porcine pericardium, aortic homografts, reconstituted collagen, and the like suitable for invasive, or in-dwelling use in a human or animal body.

摘要翻译： 生物材料用有机化学试剂二甲基亚砜处理，以延缓体内钙化的发生。 如此处理的生物材料在长时间内变得耐钙化，对宿主没有已知的有害影响。 该方法包括将天然生物材料在化学试剂中，在戊二醛固定之前或之后，在室温或高达60℃的温度下孵育。可用该方法处理的材料包括用于生物预处理的生物材料， 例如猪主动脉瓣，猪主动脉瓣小叶，牛，马和猪心包膜，主动脉同种移植物，重建胶原蛋白等，适合在人体或动物身上进行侵入或住宅使用。