公开(公告)号：US20050175606A1

公开(公告)日：2005-08-11

申请号：US10474345

申请日：2002-04-10

Applicant: Hua-Liang Huang ,  Ju-Long Cheng ,  Xiang-Bin Wang ,  Li-Ping Song ,  Zhong Zhang ,  Qing Lin ,  Ying Gu

Inventor： Hua-Liang Huang ,  Ju-Long Cheng ,  Xiang-Bin Wang ,  Li-Ping Song ,  Zhong Zhang ,  Qing Lin ,  Ying Gu

IPC: C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K7/64 ,  C07K16/18 ,  C07K16/28 ,  C07K16/30 ,  C07K16/32 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/13 ,  C12N15/63

CPC classification number: C07K16/30 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/2818 ,  C07K2317/31 ,  C07K2317/55

Abstract: The invention provides a cyclic single-chain trispecific antibody against human tumor. It comprises three parts. The first part is an anti-tumor Fab antibody, an anti-tumor single-domain antibody or an scFv. The second part is a reshaped Fab antibody against human CD3, a reshaped single-domain antibody against human CD3 or a reshaped scFv against human CD3. The third part is a reshaped Fab antibody against human CD28, a reshaped single-domain antibody against human CD28 or a reshaped scFv against human CD28. The present invention also offers the DNA sequence coding for this trispecific antibody, expression vectors containing this DNA sequence and host cells (E. coli) containing the vectors.

Abstract translation: 本发明提供了抗人肿瘤的环状单链三特异性抗体。 它包括三部分。 第一部分是抗肿瘤Fab抗体，抗肿瘤单结构域抗体或scFv。 第二部分是针对人CD3的重塑Fab抗体，针对人CD3的重构单域抗体或针对人CD3的重构scFv。 第三部分是针对人CD28的重塑Fab抗体，针对人CD28的重构单结构域抗体或针对人CD28的重构scFv。 本发明还提供编码该三特异性抗体的DNA序列，含有该DNA序列的表达载体和含有该载体的宿主细胞（大肠杆菌）。