公开(公告)号：US10029213B2

公开(公告)日：2018-07-24

申请号：US13583309

申请日：2011-03-10

申请人： Rahul Dixit ,  Mukesh Kapila ,  Yanling Wu

发明人： Rahul Dixit ,  Mukesh Kapila ,  Yanling Wu

IPC分类号： B01D63/06 ,  B01D65/02

摘要： A system for separating solids from fluid including a solid-laden fluid may include a base fluid, a first separator configured to receive the solid-laden fluid and separate the fluid into a solids portion and an effluent, and a membrane separator configured to receive the effluent and separate the effluent into a permeate and a concentrate. A method for separating solids from fluid may include obtaining a solid-laden fluid, wherein the solid-laden fluid comprises a base fluid, feeding the solid-laden fluid through a centrifuge, removing at least a portion of high gravity solids from the solid-laden fluids, flowing the solid-laden fluid through a membrane separator, removing at least a portion of low gravity solids from the solid-laden fluid, and collecting a permeate from the membrane separator.

公开(公告)号：US20130264284A1

公开(公告)日：2013-10-10

申请号：US13583309

申请日：2011-03-10

申请人： Rahul Dixit ,  Mukesh Kapila ,  Yanling Wu

发明人： Rahul Dixit ,  Mukesh Kapila ,  Yanling Wu

IPC分类号： B01D63/06

CPC分类号： B01D63/06 ,  B01D65/02 ,  B01D2311/04 ,  B01D2311/08 ,  B01D2321/04 ,  B01D2311/2676 ,  B01D2311/12

摘要： A system for separating solids from fluid including a solid-laden fluid including a base fluid, a first separator configured to receive the solid-laden fluid and separate the fluid into a solids portion and an effluent, and a membrane separator configured to receive the effluent and separate the effluent into a permeate and a concentrate is disclosed. A method for separating solids from fluid including obtaining a solid-laden fluid, wherein the solid-laden fluid comprises a base fluid, feeding the solid-laden fluid through a centrifuge, removing at least a portion of high gravity solids from the solid-laden fluids, flowing the solid-laden fluid through a membrane separator, removing at least a portion of low gravity solids from the solid-laden fluid, and collecting a permeate from the membrane separator is also disclosed.

摘要翻译： 一种用于从包括基础流体的固体流体的流体中分离固体的系统，被配置成接收固体载体并将流体分离成固体部分和流出物的第一分离器和被配置成接收流出物的膜分离器 并将流出物分离成渗透物，并且公开了浓缩物。 一种用于从流体中分离固体的方法，包括获得固体流体，其中固体流体包括基础流体，通过离心机供给载有固体的流体，从固体载体中去除至少一部分高重力固体 流体，使固体载体流过膜分离器，从固体载体流体中除去至少一部分低重力固体，以及从膜分离器收集渗透物。

公开(公告)号：US20090246212A1

公开(公告)日：2009-10-01

申请号：US12088812

申请日：2006-09-29

申请人： Takaaki Koshiba ,  Atsushi Ito ,  Shimon Sakaguchi ,  Koichi Tanaka ,  Yanling Wu ,  Mayuko Narumoto

发明人： Takaaki Koshiba ,  Atsushi Ito ,  Shimon Sakaguchi ,  Koichi Tanaka ,  Yanling Wu ,  Mayuko Narumoto

IPC分类号： A61K39/00 ,  C12Q1/02 ,  C12N5/00 ,  C12N5/06 ,  C12Q1/68

CPC分类号： G01N33/505 ,  C12N5/0636

摘要： The present invention provides a screening method for a compound capable of inducing regulatory T cells, comprising the following steps:(1) a step for culturing CD4+ T cells having a naïve phenotype in the presence of a test compound, and isolating T cells from the culture product; (2) a step for evaluating the immunosuppressive function of the T cells isolated in the step (1); (3) a step for obtaining the foregoing test compound as a compound capable of inducing regulatory T cells if the results of the evaluation in the step (2) show that the T cells isolated in the step (1) have immunosuppressive function. The present invention also provides a method of producing regulatory T cells, comprising culturing CD4+ T cells having a naïve phenotype in the presence of a rapamycin compound to obtain regulatory T cells. The regulatory T cell produced by the method can be used as an immunomodulator for the prophylaxis or treatment of the rejection in organ transplantation, an allergic disease, an autoimmune disease, a graft-versus-host disease (GVHD), infertility and the like.

摘要翻译： 本发明提供了能够诱导调节性T细胞的化合物的筛选方法，包括以下步骤：（1）在试验化合物存在下培养具有初始表型的CD4 + T细胞的步骤， 文化产品; （2）评价步骤（1）中分离的T细胞的免疫抑制功能的步骤。 （3）如果步骤（2）中的评价结果​​表明在步骤（1）中分离的T细胞具有免疫抑制功能，则获得作为能诱导调节性T细胞的化合物的上述试验化合物的步骤。 本发明还提供了一种产生调节性T细胞的方法，包括在雷帕霉素化合物存在下培养具有初始表型的CD4 + T细胞以获得调节性T细胞。 通过该方法产生的调节性T细胞可用作免疫调节剂，用于预防或治疗器官移植排斥反应，过敏性疾病，自身免疫性疾病，移植物抗宿主病（GVHD），不育症等。