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1.
公开(公告)号:US4695539A
公开(公告)日:1987-09-22
申请号:US516241
申请日:1983-07-22
申请人: Yoshitsugu Sakata , Yoshibonu Miyashita , Tadashi Hamanaka , Hiroyuki Kodera , Yutaka Miki , Kazuhiko Yamanishi , Toshiro Hanada
发明人: Yoshitsugu Sakata , Yoshibonu Miyashita , Tadashi Hamanaka , Hiroyuki Kodera , Yutaka Miki , Kazuhiko Yamanishi , Toshiro Hanada
CPC分类号: C12Q1/60 , C12Q1/26 , C12Q1/54 , C12Q1/62 , Y10S435/81
摘要: A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
摘要翻译: 样品中的底物可以通过测量通过用相应于所要测定的底物的特定氧化酶处理样品而产生的比色超氧化物离子来测定,所述氧化酶处理和所测得的超氧化物离子是通过使用包含( a)氧化酶,(b)过氧化物酶,(c)苯酚和/或胺,(d)硫醇化合物,(e)待还原的着色剂,以及必要时(f)螯合剂。