公开(公告)号：US5622849A

公开(公告)日：1997-04-22

申请号：US517545

申请日：1995-08-21

申请人： Yuichi Fusho ,  Yoshihiro Yajima

发明人： Yuichi Fusho ,  Yoshihiro Yajima

IPC分类号： C12N9/08 ,  C12R1/125

CPC分类号： C12N9/0065 ,  Y10S435/839

摘要： A microbial catalase having a catalase activity at 0.degree. C. of 95% or more of its catalase activity at 30.degree. C., when measured at pH 7, and a process for producing the same. The microbial catalase preferably has (1) an operative temperature of 0.degree. to 60.degree. C. and an optimum temperature of 0.degree. to 30.degree. C., when measured within the range of from 0.degree. to 60.degree. C., (2) an optimum pH of 7 to 10, (3) a resistance to 10 mM potassium fluoride, (4) a molecular weight is 65,000.+-.3,000, when measured by SDS polyacrylamide gel electrophoresis, and (5) an isoelectric point is about 4.8, when measured by isoelectric focusing.

摘要翻译： 当在pH7下测量时，在30℃具有过氧化氢酶活性的微生物过氧化氢酶，其在30℃下的过氧化氢酶活性为95％或更高，其制备方法为。 微生物过氧化氢酶优选具有（1）0至60℃的操作温度和0至30℃的最佳温度，当在0至60℃的范围内测量时，（2） 最佳pH为7〜10，（3）耐10mM氟化钾，（4）分子量为65,000 +/- 3,000，当通过SDS聚丙烯酰胺凝胶电泳测定时，（5）等电点约为4.8 ，通过等电聚焦测量。

公开(公告)号：US4007561A

公开(公告)日：1977-02-15

申请号：US613300

申请日：1975-09-15

申请人： Masami Okano ,  Morito Sadamura ,  Muraichi Iwasaki ,  Takeshi Suzuki ,  Yasushi Utunomiya ,  Yoshihiro Yajima ,  Yozi Yamauchi ,  Yutaka Terawaki

发明人： Masami Okano ,  Morito Sadamura ,  Muraichi Iwasaki ,  Takeshi Suzuki ,  Yasushi Utunomiya ,  Yoshihiro Yajima ,  Yozi Yamauchi ,  Yutaka Terawaki

IPC分类号： B24B15/02 ,  B24B15/08 ,  B24B23/08 ,  B24B9/00 ,  B24B23/00

CPC分类号： B24B23/08 ,  B24B15/02

摘要： The disclosed device comprises a supporting disc, a sleeve centrally pendent from the disc, a hollow driven shaft rotatably and axially slidably extending through the disc and sleeve, and a lapping disc fixed to that end of the shaft extending from the sleeve. With the supporting disc disposed upon a valve body including a valve seat to be lapped, the lapping disc can contact the valve seat to fit it during the rotation of the shaft. With the lapping disc raised from the lapped valve seat, a wiper on the disc is actuated to wipe the latter. The seat can be observed through a periscope type observation glass extending through the hollow shaft.

摘要翻译： 所公开的装置包括支撑盘，从盘中心悬挂的套筒，可旋转并且轴向可滑动地延伸穿过盘和套筒的中空从动轴，以及固定到从套筒延伸的轴的端部的研磨盘。 当支撑盘设置在包括要研磨的阀座的阀体上时，研磨盘可以在轴的旋转期间接触阀座以使其适配。 随着研磨盘从搭接的阀座上升起，盘上的擦拭器被致动以擦拭盘。 可以通过延伸穿过中空轴的潜望镜型观察玻璃观察座椅。

公开(公告)号：US5486467A

公开(公告)日：1996-01-23

申请号：US354721

申请日：1994-12-06

申请人： Yuichi Fusho ,  Yoshihiro Yajima

发明人： Yuichi Fusho ,  Yoshihiro Yajima

IPC分类号： C12N9/08 ,  C12R1/125 ,  C12N1/00 ,  C12N1/20

CPC分类号： C12N9/0065 ,  Y10S435/839

摘要： A microbial catalase having a catalase activity at 0.degree. C. of 95% or more of its catalase activity at 30.degree. C., when measured at pH 7, and a process for producing the same. The microbial catalase preferably has (1) an operative temperature of 0.degree. to 60.degree. C. and an optimum temperature of 0.degree. to 30.degree. C., (2) an optimum pH of 7 to 10, (3) a resistance to 10 mM potassium fluoride, (4) a molecular weight is 65,000.+-.3,000, when measured by SDS polyacrylamide gel electrophoresis, and (5) an isoelectric point is about 4.8, when measured by isoelectric focusing. The catalase is obtainable from Bacillus subtilis IAM 1206 (FERM BP-4844) or a mutant strain thereof.

摘要翻译： 当在pH7下测量时，在30℃具有过氧化氢酶活性的微生物过氧化氢酶，其在30℃下的过氧化氢酶活性为95％或更高，其制备方法为。 微生物过氧化氢酶优选具有（1）0至60℃的操作温度和0至30℃的最适温度，（2）最佳pH为7至10，（3）耐10 mM氟化钾，（4）当用SDS聚丙烯酰胺凝胶电泳测定时，其分子量为65,000 +/- 3,000，（5）通过等电聚焦测定时，等电点约为4.8。 过氧化氢酶可从枯草芽孢杆菌IAM 1206（FERM BP-4844）或其突变株获得。