公开(公告)号：US20140170772A1

公开(公告)日：2014-06-19

申请号：US14235654

申请日：2012-07-24

申请人： Youichi Ide ,  Tomonori Kaneko ,  Hidetaka Ninomiya

发明人： Youichi Ide ,  Tomonori Kaneko ,  Hidetaka Ninomiya

IPC分类号： G01N33/544

CPC分类号： G01N33/544 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2333/42

摘要： [Problem] An object of the present invention is to provide a method by which the amount of a very small amount of analyte (in particular, one having a sugar chain) can be measured with a high accuracy and a device which is used for said measurement method.[Means for Solution] The method for measuring the amount of an analyte according to the present invention is characterized in that a lectin labeled with a fluorescent dye, preferably said lectin whose dissociation rate constant [kd] is from 1.0×10−6 to 1.0×10−3 (S−1), is used as a secondary antibody in a sandwich assay using a surface plasmon excitation enhanced fluorescence spectroscopy [SPFS; Surface Plasmon-field enhanced Fluorescence Spectroscopy].

摘要翻译： 本发明的目的在于提供一种能够高精度地测定非常少量的分析物（特别是具有糖链的分析物）的量的方法，以及用于所述 测量方法。 溶液的测定方法本发明的分析物的量的测定方法的特征在于，用荧光染料，优选解离速度常数[kd]为1.0×10 -6〜1.0的所述凝集素的凝集素 ×10-3（S-1）用作夹心测定中的二抗，使用表面等离子体激发增强荧光光谱[SPFS; 表面等离子体增强荧光光谱法。