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    MODIFIED LECTIN DERIVED FROM WISTERIA FLORIBUNDA
    4.
    发明申请
    MODIFIED LECTIN DERIVED FROM WISTERIA FLORIBUNDA 有权
    来自WISTERIA FLORIBUNDA的改良的LECTIN

    公开(公告)号：US20150329602A1

    公开(公告)日：2015-11-19

    申请号：US14654223

    申请日：2013-12-18

    申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

    发明人： Takashi Sato ,  Yasunori Chiba ,  Hiroaki Tateno ,  Hiroyuki Kaji ,  Masanori Goto ,  Hisashi Narimatsu

    IPC分类号： C07K14/42 ,  G01N33/53

    CPC分类号： C07K14/42 ,  G01N33/5308 ,  G01N2333/42 ,  G01N2400/00

    摘要： [Problem] The purpose of the present invention is to stably supply high-quality and highly uniform Wisteria floribunda agglutinin (WFA) that recognizes biologically important sugar-chain markers, to elucidate the sugar-chain recognition activity in detail, and to furthermore increase the specificity of the sugar-chain recognition activity. [Solution] The present invention involves the development of a technique for cloning genes for coding Wisteria floribunda agglutinin (WFA) and producing recombinant WFA having the same sugar-chain recognition activity as natural WFA from transformed bacteria. Natural WFA is reduced to thereby manufacture a reduced WFA monomer for specifically recognizing terminal GalNAc residue. A recombinant monomer WFA for recognizing LDN (GalNAcβ1, 4GlcNAc) sugar chain, which is important as a diagnostic marker among sugar chains having a terminal GalNAc residue, is manufactured by introducing cysteine mutation to recombinant WFA or by C-terminal-side amino acid deletion.

    摘要翻译： [问题]本发明的目的是稳定地提供识别生物重要的糖链标记物的高品质和高度均匀的紫藤凝集素（WFA），以详细阐明糖链识别活性，并进一步增加 糖链识别活性的特异性。 [解决方案]本发明涉及开发克隆用于编码Wisteria floribunda凝集素（WFA）的基因的克隆技术，并且生产与来自转化细菌的天然WFA具有相同糖链识别活性的重组WFA。 减少天然WFA，从而制造用于特异性识别末端GalNAc残基的还原的WFA单体。 通过将半胱氨酸突变引入重组WFA或C末端侧氨基酸来制备重要单体WFA，其用于识别LDN（GalNAc＆bgr; 1,4GlcNAc）糖链，其作为具有末端GalNAc残基的糖链中的诊断标记是重要的 酸缺失。

    ENGINEERED MICROBE-TARGETING MOLECULES AND USES THEREOF
    6.
    发明申请
    ENGINEERED MICROBE-TARGETING MOLECULES AND USES THEREOF 有权
    工程微生物靶向分子及其用途

    公开(公告)号：US20140227723A1

    公开(公告)日：2014-08-14

    申请号：US14233553

    申请日：2012-07-18

    申请人： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

    发明人： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

    IPC分类号： C07K16/12 ,  G01N33/569 ,  C07K14/42

    CPC分类号： C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  C07K2319/33 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/53 ,  Y02A50/58

    摘要： Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

    摘要翻译： 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包含与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可以用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。

    NON COVALENT MOLECULAR STRUCTURE, COMPRISING A PYRENE BASED GLYCOCONJUGATE, DEVICE COMPRISING THE SAME AND ITS USE FOR DETECTION OF LECTIN
    7.
    发明申请
    NON COVALENT MOLECULAR STRUCTURE, COMPRISING A PYRENE BASED GLYCOCONJUGATE, DEVICE COMPRISING THE SAME AND ITS USE FOR DETECTION OF LECTIN 审中-公开
    非共价分子结构，包含基于聚苯乙烯的甘油三酯，其包含其的装置及其用于检测LECTIN

    公开(公告)号：US20140147938A1

    公开(公告)日：2014-05-29

    申请号：US14131330

    申请日：2011-07-12

    申请人： Anne Imberty ,  Sébastien Vidal ,  Alxander Star

    发明人： Anne Imberty ,  Sébastien Vidal ,  Alxander Star

    IPC分类号： G01N33/543 ,  G01N33/566 ,  C07H15/26

    CPC分类号： G01N33/566 ,  C07H15/26 ,  G01N33/5438 ,  G01N2333/42 ,  G01N2333/4724

    摘要： The present invention relates to a non covalent molecular structure comprising a carbon nanostructure and a pyrene based glycoconjugate (I) which is linked to the said carbon nanostructure by a non covalent link, the said glycoconjugate (I) having the formula: wherein B is a group which is present on any of the ten carbon atoms of the pyrene structure represented in (I) susceptible to bear a substituent, and is represented by the following group: —(CH2)n-CO—NH-A, wherein n is an integer from 1 to 9, A is a group of formula: The present invention also relates to an electronic device comprising the said non covalent molecular structure, and to the use of this device for the detection of a lectin involved in bacterial or viral infections. Thus the invention also relates to a method for detecting the presence of a lectin in a sample to be analysed.

    摘要翻译： 本发明涉及包含碳纳米结构和芘基糖缀合物（I）的非共价分子结构，其通过非共价键与所述碳纳米结构连接，所述糖缀合物（I）具有下式：其中B是 存在于易于承载取代基的（I）中表示的芘结构的十个碳原子中的任何一个的基团，并且由以下基团表示： - （CH 2）n -CO-NH-A，其中n是 A为1至9的整数，A为下式的基团：本发明还涉及包含所述非共价分子结构的电子器件，以及该装置用于检测涉及细菌或病毒感染的凝集素的用途。 因此，本发明还涉及用于检测待分析样品中凝集素的存在的方法。

    METHODS FOR SCREENING FOR THERAPEUTIC MOLECULES AND USE OF THE MOLECULES THEREFROM
    10.
    发明申请
    METHODS FOR SCREENING FOR THERAPEUTIC MOLECULES AND USE OF THE MOLECULES THEREFROM 审中-公开
    用于筛选治疗分子的方法和其分子的使用

    公开(公告)号：US20100104572A1

    公开(公告)日：2010-04-29

    申请号：US12447581

    申请日：2007-11-01

    申请人： Sylvie Luria

    发明人： Sylvie Luria

    IPC分类号： C40B30/04 ,  A61K39/395 ,  G01N33/53 ,  C07K16/00 ,  A61P35/00

    CPC分类号： G01N33/5011 ,  G01N33/57423 ,  G01N2333/42 ,  G01N2333/4724

    摘要： The present invention relates in general to the field of cancer diagnostics and treatment. In particular, the present invention provides methods for identifying diagnostic and therapeutic molecules that bind to a carbohydrate antigen on the surface of a cancer cell and induce cell death, to pharmaceutical compositions comprising the molecules and uses thereof.

    摘要翻译： 本发明一般涉及癌症诊断和治疗领域。 特别地，本发明提供了鉴定与癌细胞表面上的碳水化合物抗原结合并诱导细胞死亡的诊断和治疗分子的方法，包括该分子的药物组合物及其用途。