公开(公告)号：US20060154303A1

公开(公告)日：2006-07-13

申请号：US11331959

申请日：2006-01-13

申请人： Toru Myogadani ,  Youji Ebe ,  Masao Yamada ,  Jun Hirabayashi

发明人： Toru Myogadani ,  Youji Ebe ,  Masao Yamada ,  Jun Hirabayashi

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/54373 ,  G01N21/6452 ,  G01N21/648 ,  G01N33/582

摘要： In the case where many interactional substances are immobilized on the front surface side of a light transmitting plate capable of generating evanescent waves on the front surface, and where the fluorescence levels in the interactions between the interactional substances and a fluorescence labeled test substance are detected in a liquid layer according to a conventional method, the detection sensitivity and S/N ratio are low due to the background noise. Furthermore, it is necessary to perform a gel filtration purification step for the purpose of removing an excessive amount of the fluorescence dye used for labeling a test substance before the interactions between the fluorescence labeled test substance and the interactional substances are performed. To solve these problems, this invention proposes the above-mentioned method for detecting the evanescent wave excited fluorescence comprising the step of adding a black colorant, for example, a pigment based colorant mainly containing fine particles such as Bokuju.

摘要翻译： 在许多相互作用物质被固定在能够在前表面上产生ev逝波的透光板的前表面侧，并且其中在相互作用的物质和荧光标记的测试物质之间的相互作用中的荧光水平被检测到的情况下 根据常规方法的液体层，由于背景噪声，检测灵敏度和S / N比都较低。 此外，为了在进行荧光标记的测试物质和相互作用物质之间的相互作用之前去除用于标记测试物质的过量的荧光染料，需要进行凝胶过滤纯化步骤。 为了解决这些问题，本发明提出了上述用于检测ev逝波激发荧光的方法，其包括添加黑色着色剂的步骤，例如主要含有诸如Bokuju的微粒的颜料基着色剂。