公开(公告)号：US07160689B2

公开(公告)日：2007-01-09

申请号：US10163614

申请日：2002-06-07

申请人： Yuko Matsumoto ,  Yumiko Kobayashi ,  Junichi Okada

发明人： Yuko Matsumoto ,  Yumiko Kobayashi ,  Junichi Okada

IPC分类号： G01N33/53

CPC分类号： G01N1/10 ,  A61B10/0051 ,  B01L3/5029 ,  B01L3/505 ,  G01N2001/028 ,  G01N2333/315

摘要： In a pretreatment instrument and a pretreatment method of saliva, used for identification and quantitation of Streptococcus mutans in saliva by the immunochromatographic method utilizing an antigen-antibody reaction, the instrument includes a swab and a mixing container for saliva and a treatment liquid, the swab having a stick and a soft synthetic resin-made sponge capable of absorbing a predetermined amount or more of saliva, and the mixing container being made of a transparent or translucent soft synthetic resin and comprising a bag-like portion formed integrally with and continuously to a constricted portion as the end of a tapering introduction portion having an opening thoroughly larger than the sponge, wherein the constricted portion and the bag-like portion have elasticity such that the sponge can be squashed by a finger pressure in a state that the sponge is inserted therein; the constricted portion has a width such that a pressure can be applied by fingers and has a shape such that when the sponge is taken out in a squashed state by the finger pressure, a minimum amount necessary for the pretreatment or more of the saliva can be squeezed out.

摘要翻译： 在使用抗原 - 抗体反应的免疫色谱法在唾液的预处理仪器和预处理方法中，用于鉴别和定量唾液中的变形链球菌，仪器包括用于唾液和处理液的拭子和混合容器，拭子 具有能够吸收规定量以上的唾液的棒状物和软质合成树脂制海棉，所述混合容器由透明或半透明的软质合成树脂制成，并且包括一体形成的袋状部分， 收缩部分作为具有比海绵充分大的开口的锥形引入部分的端部，其中收缩部分和袋状部分具有弹性，使得海绵可以在海绵被插入的状态下被手指压力挤压 其中 收缩部分具有能够通过手指施加压力的宽度，并且具有这样的形状，使得当通过手指压力以压扁状态取出海绵时，预处理所需的最小量或更多的唾液可以是 挤出。

公开(公告)号：US06897037B2

公开(公告)日：2005-05-24

申请号：US10132474

申请日：2002-04-26

申请人： Junichi Okada ,  Yumiko Kobayashi

发明人： Junichi Okada ,  Yumiko Kobayashi

IPC分类号： G01N31/22 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  C12Q1/04 ,  C12N1/00

CPC分类号： G01N33/56944 ,  Y10S435/885 ,  Y10S435/975

摘要： To provide a pre-treatment kit for saliva and a pre-treatment method for saliva for identification and quantitation of mutans streptococci in human saliva by the immunochromatographic method, which can eliminate mucin in saliva and prevent mutans streptococci from chaining and aggregation in a simple method, the pre-treatment kit is constructed of (A) an aqueous solution containing sodium hydroxide, (B) a tris(hydroxymethyl)aminomethane buffer solution containing tartaric acid and/or citric acid, and (C) a nonionic surfactant and/or an amphoteric surfactant, wherein the component (C) is previously mixed with or prepared separately from the component (A) and/or the component (B), and further (D) a pH indicator having a color transition range of pH 5 to 9 is previously mixed with or prepared separately from the component (A) or the component (A) having the component (C) previously mixed therewith, and/or the component (B) or the component (B) having the component (C) previously mixed therewith. According the pre-treatment method, the respective components of the pre-treatment kit is added dropwise into and mixed with saliva in an arbitrary order.

摘要翻译： 提供唾液预处理试剂盒和唾液预处理方法，用于通过免疫色谱法鉴定和定量人唾液中变形链球菌，可以消除唾液中的粘蛋白，并以简单的方法预防变形链球菌的链接和聚集 ，所述预处理试剂盒由（A）含有氢氧化钠的水溶液，（B）含有酒石酸和/或柠檬酸的三（羟甲基）氨基甲烷缓冲溶液和（C）非离子表面活性剂和/或 两性表面活性剂，其中组分（C）预先与组分（A）和/或组分（B）混合或制备，进一步（D）具有pH5至9的颜色过渡范围的pH指示剂为 预先与预先混合有组分（C）的组分（A）或组分（A）混合或制备，和/或组分（B）或组分（B）预先混合的组分（B）或组分 的 rewith。 根据预处理方法，将预处理试剂盒的各成分按任意顺序逐滴加入唾液中并与其混合。