公开(公告)号：US6063912A

公开(公告)日：2000-05-16

申请号：US129888

申请日：1998-08-06

申请人： Jong-Yoon Chun ,  Yun-Jeong Han

发明人： Jong-Yoon Chun ,  Yun-Jeong Han

IPC分类号： C07K14/47 ,  C07H21/04

CPC分类号： C07K14/4702

摘要： Expression of the polynucleotide molecule, Psx, is restricted to placenta, and in particular, to placenta trophoblast cell layers during embryogenesis. The expression pattern of Psx is exploited to detect trophoblast specific lineages, such as labyrinthine trophoblast layer and giant cells. The invention provides an isolated DNA molecule encoding Psx protein, and chimeric constructs, vector and host cells containing the isolated DNA. Also provided is a method for identifying putative abortion-inducing agents, which may offer an alternative to surgical abortion.

摘要翻译： 多核苷酸分子Psx的表达限于胎盘，特别是胚胎发生期间对胎盘滋养层细胞层的表达。 Psx的表达模式被用于检测滋养层特异性谱系，如迷路滋养层和巨细胞。 本发明提供了编码Psx蛋白的分离的DNA分子和含有分离的DNA的嵌合构建体，载体和宿主细胞。 还提供了用于鉴定推定的流产诱导剂的方法，其可以提供手术流产的替代方案。

公开(公告)号：US20070136897A1

公开(公告)日：2007-06-14

申请号：US11247236

申请日：2005-10-12

申请人： Jeong-II Kim ,  Yun-Jeong Han ,  Seong-Hee Kim ,  Pill-Soon Song

发明人： Jeong-II Kim ,  Yun-Jeong Han ,  Seong-Hee Kim ,  Pill-Soon Song

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N9/1229 ,  C12N15/8261 ,  C12N15/8271 ,  Y02A40/146

摘要： The present invention includes modified Arabidopsis Nucleoside Di-Phosphate Kinase 2 (NDPK2) nucleic acid molecules whose enzymatic activity have been increased (i.e. hyperactive). NDPKs are ubiquitous housekeeping enzymes that catalyze the transfer of γ-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP), and also multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. In plants, NDPKs are reported to play a key role in the signaling of both stress and light. Among three NDPKs (NDPK1, NDPK2, NDPK3) in a model plant, Arabidopsis thaliana, NDPK2 was reported as a positive signal transducer of phytochrome-mediated plant light signaling and to regulate cellular redox state, which enhances multiple stress tolerance in transgenic plants. Thus, the plants with the hyperactive NDPK2 are expected to possess higher efficiency of light utilization and enhanced tolerance to various environmental stresses such as cold, salt, and oxidative stresses. Since abiotic stress is one of the most important factors to limit the productivity of many crops, the hyperactive NDPK2 can be used for the development of high-yielding multiple stress tolerant plants with higher efficiency of light utilization. In this invention, several hyperactive NDPK2 were generated by C-terminal deletion and site-directed mutagenesis. Therefore, the present invention can be utilized to develop multiple stress tolerant and efficiently light-utilizing plants, which can eventually increase crop yields. The invention also includes plants having at least one cell expressing the modified NDPK2, vectors comprising at least one portion of the modified NDPK2 nucleic acids, and methods using such vectors for producing plants with enhanced light sensitivity and stress tolerance.

公开(公告)号：US20060260009A1

公开(公告)日：2006-11-16

申请号：US11129459

申请日：2005-05-16

申请人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

发明人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.

摘要翻译： 本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。 具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。 由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。 因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。 在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。 具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。 因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。 本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。

公开(公告)号：US07285652B2

公开(公告)日：2007-10-23

申请号：US11129459

申请日：2005-05-16

申请人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

发明人： Jeong-Il Kim ,  Yun-Jeong Han ,  Pill-Soon Song

IPC分类号： C07H21/04

CPC分类号： C12N15/8261 ,  C07K14/415 ,  Y02A40/146

摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which Pr absorption spectra have been shifted to longer wavelength (i.e. bathochromism or red-shift). The plants with the bathochromic phytochromes are expected to respond to canopy and shade conditions for growth and development with greater efficiency than the plants with wild-type phytochrome (i.e. suppression of shade avoidance reactions in plants). Since the shade avoidance reactions in plants induce a rapid and dramatic increase in the extension growth of stems and petioles at the expense of leaf growth, storage organ production, and reproductive development, it causes significant losses of crop yields. Thus, the bathochromic phytochromes that utilize the shade light efficiently would suppress the shade avoidance reactions in plants, giving plants the tolerance to shade. In this invention, several bathochromic phytochromes were generated by site-directed mutagenesis in the region of bilin lyase domain in plant PHYA, and their ability to suppress the shade avoidance reactions were examined by transforming the bathochromic phytochromes into PHYA deficient Arabidopsis thaliana (ecotype col-0). The transgenic plants with the bathochromic phytochromes showed significantly increased shade tolerance compared to wild-type plants and transgenic plants with wild-type phytochromes. Therefore, the present invention can be utilized to suppress plants' shade avoidance that is one of major causes to induce crop-yield losses, and ultimately to generate shade tolerant plants with higher yields. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with shade tolerance.

摘要翻译： 本发明包括修饰的植物色素A（PHYA）核酸分子，其中Pr吸收光谱已经转移到更长的波长（即，红移或红移）。 具有红色色素植物色素的植物预期对具有野生型植物色素的植物（即抑制植物中的树荫回避反应）的效率比用于生长和发育的冠层和阴凉条件更有效。 由于植物中的避光反应以叶片生长，储存器官生产和繁殖发育为代价诱导茎和叶柄的延伸生长快速和显着地增加，导致作物产量的显着损失。 因此，有效利用遮光效果的红色植物色素可以抑制植物的避光反应，使植物具有遮荫性。 在本发明中，通过在植物PHYA中的胆固醇裂解酶结构域中的定点诱变产生了几种红色植物色素，并且通过将红色染色体变成PHYA缺陷拟南芥（生态型col- 0）。 具有红移植物色素的转基因植物与野生型植物和具有野生型植物色素的转基因植物相比，显示明显增加的遮荫耐受性。 因此，本发明可以用于抑制作物诱导作物产量损失的主要原因之一的植物避光，最终产生具有较高产量的耐荫植物。 本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有阴性耐受性的植物的方法。