公开(公告)号：US07485705B2

公开(公告)日：2009-02-03

申请号：US10563702

申请日：2004-07-13

申请人： Yuriko Fukuoka ,  Ryo Sakamoto ,  Munetaka Ishiyama

发明人： Yuriko Fukuoka ,  Ryo Sakamoto ,  Munetaka Ishiyama

IPC分类号： C09B44/08 ,  C12Q1/26 ,  C12Q1/32

CPC分类号： C07D257/04 ,  C12Q1/32

摘要： The purpose of the present invention is to provide a water-soluble tetrazolium compound that will form a water-soluble formazan exhibiting long-wavelength absorption and is stable in aqueous solution for a long period and suitable for the quantitative analysis of dehydrogenases or substrates thereof. Disclosed is a water soluble tetrazolium compound expressed by the following general formula (1): wherein each of R1 to R19 independently represents hydrogen atom; nitro group; sulfonate group or alkyl, alkoxy, sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; provided that each of at least two of R1 to R19 independently represents sulfonate group; or sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; and M represents an alkali metal or ammonium ion.

摘要翻译： 本发明的目的是提供一种水溶性四唑鎓化合物，其将形成长期吸收的水溶性甲for，并且在水溶液中长时间稳定并适用于脱氢酶或其底物的定量分析。 公开了由以下通式（1）表示的水溶性四唑化合物：其中R 1至R 19各自独立地表示氢原子; 硝基; 磺酸酯基或具有1至4个碳原子的烷基，烷氧基，磺基烷基或磺基烷氧基; 条件是R 1至R 19中的至少两个独立地表示磺酸基; 或具有1至4个碳原子的磺基烷基或磺基烷氧基; M表示碱金属或铵离子。

公开(公告)号：US20070111274A1

公开(公告)日：2007-05-17

申请号：US10563702

申请日：2004-07-13

申请人： Yuriko Fukuoka

发明人： Yuriko Fukuoka

IPC分类号： C12Q1/26 ,  C09B44/00

CPC分类号： C07D257/04 ,  C12Q1/32

摘要： The purpose of the present invention is to provide a water-soluble tetrazolium compound that will form a water-soluble formazan exhibiting long-wavelength absorption and is stable in aqueous solution for a long period and suitable for the quantitative analysis of dehydrogenases or substrates thereof. Disclosed is a water soluble tetrazolium compound expressed by the following general formula (1): wherein each of R1 to R19 independently represents hydrogen atom; nitro group; sulfonate group or alkyl, alkoxy, sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; provided that each of at least two of R1 to R19 independently represents sulfonate group; or sulfoalkyl or sulfoalkyloxy groups having 1 to 4 carbon atoms; and M represents an alkali metal or ammonium ion.

摘要翻译： 本发明的目的是提供一种水溶性四唑鎓化合物，其将形成长期吸收的水溶性甲for，并且在水溶液中长时间稳定并适用于脱氢酶或其底物的定量分析。 公开了由以下通式（1）表示的水溶性四唑化合物：其中R 1至R 20各自独立地表示氢原子; 硝基; 磺酸酯基或具有1至4个碳原子的烷基，烷氧基，磺基烷基或磺基烷氧基; 条件是R 1至R 19中的至少两个独立地表示磺酸基; 或具有1至4个碳原子的磺基烷基或磺基烷氧基; M表示碱金属或铵离子。