公开(公告)号：US09663560B2

公开(公告)日：2017-05-30

申请号：US13445696

申请日：2012-04-12

申请人： Ibrokhim Y. Abdurakhmonov ,  Zabardast T. Buriev ,  Abdusattor Abdukarimov ,  Sukumar Saha ,  Johnie N. Jenkins ,  Alan E. Pepper

发明人： Ibrokhim Y. Abdurakhmonov ,  Zabardast T. Buriev ,  Abdusattor Abdukarimov ,  Sukumar Saha ,  Johnie N. Jenkins ,  Alan E. Pepper

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

摘要： Improvement of fiber quality of Upland cultivars (Gossypium hirsutum), while maintaining early maturity and productivity, is a fundamental problem in conventional cotton breeding. Phytochromes play a fundamental role in plant development, flowering and cotton fiber length. Targeted RNAi of PHYA1 genes in cotton suppressed expression of PHYA1 and/or PHYB, resulting in over-expression of the remaining PHYA2/B/C/E genes. This altered expression induced a number of phytochrome-associated phenotypes, including increased root length and mass, increased anthocyanin-pigment, vigorous shoot development and vegetative growth, early flowering, early boll maturity, increased fiber length and increased seed cotton yield compared to control plants. These RNAi phenotypes were stably inherited and expressed through four generations (T0-3) and were transferable from RNAi Coker-312 plants to Upland cultivars via conventional hybridization.

公开(公告)号：US20130227723A1

公开(公告)日：2013-08-29

申请号：US13445696

申请日：2012-04-12

申请人： Ibrokhim Y. Abdurakhmonov ,  Zabardast T. Buriev ,  Abdusattor Abdukarimov ,  Sukumar Saha ,  Johnie N. Jenkins ,  Alan E. Pepper

发明人： Ibrokhim Y. Abdurakhmonov ,  Zabardast T. Buriev ,  Abdusattor Abdukarimov ,  Sukumar Saha ,  Johnie N. Jenkins ,  Alan E. Pepper

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N15/63 ,  A01H5/10 ,  A01H5/00 ,  A01H1/02 ,  C12Q1/68 ,  C07H21/04 ,  C12N5/10

CPC分类号： C07K14/415 ,  C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

摘要： Improvement of fiber quality of Upland cultivars (Gossypium hirsutum), while maintaining early maturity and productivity, is a fundamental problem in conventional cotton breeding. Phytochromes play a fundamental role in plant development, flowering and cotton fiber length. Targeted RNAi of PHYA1 genes in cotton suppressed expression of PHYA1 and/or PHYB, resulting in over-expression of the remaining PHYA2/B/C/E genes. This altered expression induced a number of phytochrome-associated phenotypes, including increased root length and mass, increased anthocyanin-pigment, vigorous shoot development and vegetative growth, early flowering, early boll maturity, increased fiber length and increased seed cotton yield compared to control plants. These RNAi phenotypes were stably inherited and expressed through four generations (T0-3) and were transferable from RNAi Coker-312 plants to Upland cultivars via conventional hybridization.

摘要翻译： 在保持早熟和生产力的同时，提高陆地品种（Gossypium hirsutum）的纤维质量是传统棉花育种中的根本问题。 植物色素在植物发育，开花和棉纤维长度方面起着至关重要的作用。 棉花中PHYA1基因的靶向RNAi抑制了PHYA1和/或PHYB的表达，导致剩余的PHYA2 / B / C / E基因过度表达。 与对照植物相比，这种改变的表达诱导了许多植物色素相关表型，包括增加的根长度和质量，增加的花青素 - 颜料，剧烈的枝条发育和营养生长，早期开花，早熟成熟，增加的纤维长度和增加的种子棉花产量 。 这些RNAi表型通过四代（T0-3）稳定遗传并表达，可通过常规杂交从RNAi Coker-312植物转移到山地品种。