公开(公告)号：US06942970B2

公开(公告)日：2005-09-13

申请号：US09952851

申请日：2001-09-14

申请人： Jorma Isola ,  Minna Tanner ,  Tero Jarvinen ,  Zuo-Rong Shi ,  Rina Wu

发明人： Jorma Isola ,  Minna Tanner ,  Tero Jarvinen ,  Zuo-Rong Shi ,  Rina Wu

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6841 ,  C12Q2563/125

摘要： The present invention provides methods for diagnosing and treating cancer, and in particular methods for determining the susceptibility of subjects suspected of having breast cancer (or known to have breast cancer) to treatment with topoisomerase II inhibitors. The present invention also provides in situ hybridization probes for specifically detecting topoIIα gene sequences.

摘要翻译： 本发明提供了用于诊断和治疗癌症的方法，特别是用于确定怀疑患有乳腺癌（或已知具有乳腺癌）的受试者对用拓扑异构酶II抑制剂进行治疗的易感性的方法。 本发明还提供用于特异性检测topoIIalpha基因序列的原位杂交探针。

公开(公告)号：US5869274A

公开(公告)日：1999-02-09

申请号：US891238

申请日：1997-07-10

申请人： Dean Tsao ,  Zuo-Rong Shi ,  Peter Luu

发明人： Dean Tsao ,  Zuo-Rong Shi ,  Peter Luu

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/537

CPC分类号： G01N33/54393 ,  G01N33/5306

摘要： The embodiments of the invention are multiple antibody immunoassay systems, and preferably immunohistochemical staining systems. In these systems the detection antibody system that detects the association of a targeting antibody (that binds to the target moiety to be detected) may also cross-react with endogenous immunoglobulins in the specimen in which the target moiety is to be detected, yielding an unreliable assay with high background levels. This problem has been overcome by a process that includes cross-linking a monovalent antibody to the endogenous antigenic determinants prior to exposure to the targeting antibody and detecting antibody system. The embodiments of the invention also include kits for use in these multiple antibody immunoassay and histochemical staining systems.

摘要翻译： 本发明的实施方案是多重抗体免疫测定系统，优选免疫组织化学染色系统。 在这些系统中，检测靶向抗体（与待检测的靶标部分结合）的检测抗体系统也可与待检测目标部分的标本中的内源性免疫球蛋白交叉反应，产生不可靠的 具有高背景水平的测定。 通过包括在暴露于靶向抗体并检测抗体系统之前将单价抗体与内源性抗原决定簇交联的方法克服了该问题。 本发明的实施方案还包括用于这些多重抗体免疫测定和组织化学染色系统的试剂盒。

公开(公告)号：US20090233803A1

公开(公告)日：2009-09-17

申请号：US11840877

申请日：2007-08-17

申请人： Zuo-Rong Shi ,  Rina Wu

发明人： Zuo-Rong Shi ,  Rina Wu

IPC分类号： C40B30/04

CPC分类号： C12Q1/6841 ,  C12Q2563/125

摘要： The present invention relates to chromogenic (calorimetric) in situ hybridization (CISH) and nucleic acid probes useful for in situ hybridization. Specifically, the present invention provides methods, kits, and compositions for performing bright field cancer diagnostics employing chromogenic in situ hybridization (e.g. to detect gene amplifications, gene translocations, and chromosome polysomy). In preferred embodiments, the present invention provides CISH methods, kits and compositions for detecting HER2 gene status.

公开(公告)号：US20090137412A1

公开(公告)日：2009-05-28

申请号：US11840860

申请日：2007-08-17

申请人： Zuo-Rong Shi ,  Rina Wu

发明人： Zuo-Rong Shi ,  Rina Wu

IPC分类号： C40B30/04

CPC分类号： C12Q1/6841 ,  C12Q2563/125

摘要： The present invention relates to chromogenic (calorimetric) in situ hybridization (CISH) and nucleic acid probes useful for in situ hybridization. Specifically, the present invention provides methods, kits, and compositions for performing bright field cancer diagnostics employing chromogenic in situ hybridization (e.g. to detect gene amplifications, gene translocations, and chromosome polysomy). In preferred embodiments, the present invention provides CISH methods, kits and compositions for detecting HER2 gene status.

摘要翻译： 本发明涉及用于原位杂交的显色（量热）原位杂交（CISH）和核酸探针。 具体地，本发明提供用于进行使用显色原位杂交（例如检测基因扩增，基因易位和染色体多重）的明场癌症诊断的方法，试剂盒和组合物。 在优选的实施方案中，本发明提供用于检测HER2基因状态的CISH方法，试剂盒和组合物。