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    Immuno-histochemical method that reduces background staining
    1.
    发明授权
    Immuno-histochemical method that reduces background staining 失效
    免疫组织化学方法，减少背景染色

    公开(公告)号：US5869274A

    公开(公告)日：1999-02-09

    申请号：US891238

    申请日：1997-07-10

    申请人： Dean Tsao ,  Zuo-Rong Shi ,  Peter Luu

    发明人： Dean Tsao ,  Zuo-Rong Shi ,  Peter Luu

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/537

    CPC分类号： G01N33/54393 ,  G01N33/5306

    摘要： The embodiments of the invention are multiple antibody immunoassay systems, and preferably immunohistochemical staining systems. In these systems the detection antibody system that detects the association of a targeting antibody (that binds to the target moiety to be detected) may also cross-react with endogenous immunoglobulins in the specimen in which the target moiety is to be detected, yielding an unreliable assay with high background levels. This problem has been overcome by a process that includes cross-linking a monovalent antibody to the endogenous antigenic determinants prior to exposure to the targeting antibody and detecting antibody system. The embodiments of the invention also include kits for use in these multiple antibody immunoassay and histochemical staining systems.

    摘要翻译： 本发明的实施方案是多重抗体免疫测定系统，优选免疫组织化学染色系统。 在这些系统中，检测靶向抗体（与待检测的靶标部分结合）的检测抗体系统也可与待检测目标部分的标本中的内源性免疫球蛋白交叉反应，产生不可靠的 具有高背景水平的测定。 通过包括在暴露于靶向抗体并检测抗体系统之前将单价抗体与内源性抗原决定簇交联的方法克服了该问题。 本发明的实施方案还包括用于这些多重抗体免疫测定和组织化学染色系统的试剂盒。