公开(公告)号：US20240210290A1

公开(公告)日：2024-06-27

申请号：US18417919

申请日：2024-01-19

Applicant: 10X Genomics, Inc.

Inventor： Stephane Claude Boutet ,  Alireza Salmanzadeh ,  Michael Gibbons

IPC: C12N15/10 ,  G01N1/34

CPC classification number: C12N15/1017 ,  G01N1/34

Abstract: Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

公开(公告)号：US20240002901A1

公开(公告)日：2024-01-04

申请号：US18312914

申请日：2023-05-05

Applicant: 10x Genomics, Inc.

Inventor： Michael Gibbons

IPC: C12Q1/48 ,  C12N9/12

CPC classification number: C12Q1/485 ,  C12N9/12

Abstract: Provided herein are reaction mixtures, compositions, systems, methods, and kits for assessing enzymatic activity. Aspects of the disclosure include use of a reactant barcoded oligonucleotide (RBO) construct comprising a first oligonucleotide comprising a reaction barcode sequence and a first linker that connects the first oligonucleotide to a first reactant, an amplification construct comprising a second oligonucleotide that is complementary to at least a portion of the first oligonucleotide and a second linker that connects the second oligonucleotide to a second reactant, and a blocking construct, comprising a third oligonucleotide that is complementary to at least a portion of the first oligonucleotide. A rate of reaction product generation may be measured to determine an enzymatic activity of a given enzyme.