公开(公告)号：US20200031875A1

公开(公告)日：2020-01-30

申请号：US16597850

申请日：2019-10-10

Applicant: ACADEMIA SINICA

Inventor： HAN-CHUNG WU ,  CHUNG-TAO TANG

IPC: C07K14/005 ,  A61K39/12 ,  C07K16/10 ,  G01N33/569 ,  A61K39/39 ,  C12N7/00

Abstract: Isolated mutant dengue virus E protein variants are disclosed. The variant comprises an amino acid sequence that is at least 80% identical to SEQ ID NO: 1 and has one or more amino acid residue substitutions at position corresponding to Asn8 (N8), Arg9 (R9), Val12 (V 12) and/or Glu13 (E13). The variant may comprise an amino acid sequence that is at least 90% identical to the SEQ ID NO: 1 and lack an infection-enhancing antibody-binding motif comprising the amino acid sequence of SEQ ID NO: 28 at domain I. An isolated nucleic acid sequence encoding the variant, a plasmid expressing the variant, a plasmid expressing a virus-like particle comprising the variant, a DNA vaccine, and a method of detecting the presence of a dengue virus in a biological sample are also disclosed.