公开(公告)号：US20150315628A1

公开(公告)日：2015-11-05

申请号：US14647816

申请日：2013-12-10

Applicant: ACADEMIA SINICA

Inventor： Che-Kun James SHEN ,  Chun-Chang CHEN

IPC: C12Q1/48 ,  C12Q1/68

CPC classification number: C12Q1/48 ,  C12Q1/68 ,  C12Q1/6897 ,  G01N2333/91011 ,  G01N2500/04 ,  C12Q2521/331 ,  C12Q2525/143

Abstract: Methods for identifying a test agent as a modulator of the active DNA demethylation activity of a DNA methyltransferase are disclosed. The method comprises: a) providing a methylated DNA; b) providing the DNA methyltransferase; c) allowing the methylated DNA to react with the DNA methyltransferase for a sufficient time to perform a demethylation reaction and generate a demethylated DNA product in the presence or absence of a test agent; d) analyzing the extent of demethylation; and d) comparing the extents of the demethylation in the presence and absence of the test agent, and thereby identify the test agent as a modulator of the DNA demethylation activity of the DNA methyltransferase.

Abstract translation: 公开了用于鉴定测试剂作为DNA甲基转移酶的活性DNA去甲基化活性的调节剂的方法。 该方法包括：a）提供甲基化DNA; b）提供DNA甲基转移酶; c）允许甲基化DNA与DNA甲基转移酶反应足够的时间进行去甲基化反应，并在试剂存在或不存在的情况下产生去甲基化的DNA产物; d）分析去甲基化程度; 和d）比较测试试剂存在和不存在时的去甲基化程度，从而鉴定作为DNA甲基转移酶的DNA去甲基化活性的调节剂的测试试剂。