公开(公告)号：US20220120757A1

公开(公告)日：2022-04-21

申请号：US17502399

申请日：2021-10-15

Applicant: ACADEMIA SINICA

Inventor： Huan-Cheng CHANG ,  Yuen-Yung HUI ,  Hsin-Hung LIN ,  Oliver Y. CHEN

IPC: G01N33/58 ,  G01N33/543 ,  G01N21/64

Abstract: The present disclosure discloses a detection method and a detection system for detecting an object of interest in a biological sample. The detection method comprises the following steps: providing a detection kit comprising a detection carrier and a reporter, wherein the detection carrier comprises a coating protein having a recognition site for binding the object of interest, the reporter including a spin luminescent material; mixing the reporter and the biological sample and loading the mixture onto the detection carrier; placing the detection carrier with the mixture of the reporter molecule and the biological sample in a time-varied physical field; irradiating a detection carrier placed in the time-varied physical field with an excitation light to excite the spin luminescent material to generate a fluorescence signal modulated by the time-varied physical field; and receiving and analyzing the fluorescence signal.

公开(公告)号：US20180340938A1

公开(公告)日：2018-11-29

申请号：US15990189

申请日：2018-05-25

Applicant: ACADEMIA SINICA

Inventor： Huan-Cheng CHANG ,  Feng-Jen HSIEH ,  Yen-Wei CHEN

IPC: G01N33/58

Abstract: This invention is a functionalized diamond crystal with high dispersibility in high ionic strength solution and/or with specific targeting ability, which comprise a diamond crystal and a fatty acid layer. The fatty acid layer works a surfactant and provides a specific targeting feature for the diamond crystal. The feature of the surfactant makes the diamond crystal being easily dispersed in biological surrounding (e.g., phosphate saline buffer) and the feature of specific targeting ability provides the diamond crystal with specific recognizing specific targets. This invention allows researchers to use diamond crystal as a marker for specific labelling.