公开(公告)号：US20220228139A1

公开(公告)日：2022-07-21

申请号：US17612635

申请日：2020-05-21

Applicant: ACADEMIA SINICA

Inventor： Kuo-Ping CHIU ,  Hsin-Chieh SHIAU ,  Zee Hong GOH

IPC: C12N15/10 ,  C12Q1/6855 ,  C12Q1/686 ,  C12N9/22

Abstract: The present invention relates to a method for amplifying and detecting ribonucleic acid (RNA) fragments. In particular, the method of the present invention comprises conversion of RNA fragments to cDNA and DNA amplification. The present invention also provides a kit for performing the method as described herein.

公开(公告)号：US20210340611A1

公开(公告)日：2021-11-04

申请号：US17283809

申请日：2019-10-09

Applicant: ACADEMIA SINICA

Inventor： Kuo-Ping CHIU

IPC: C12Q1/6855

Abstract: The present invention relates to a method for detecting nucleic acid (NA) molecules in samples. More particularly, the present invention relates to an improved digital PCR-based method for detecting specific nucleic acid sequence(s). The present invention is useful for research and diagnostic applications with increased sensitivity and accuracy. The present invention also provides a kit for performing the method for assessingnucleic acids in samples as described herein.