公开(公告)号：US20150017230A1

公开(公告)日：2015-01-15

申请号：US14381362

申请日：2013-03-01

Applicant: ACADEMIA SINICA

Inventor： Han-Chung Wu ,  Mei-Ying Liao ,  Cheng-Wei Lin

IPC: C07K16/18 ,  G01N33/574

CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/574

Abstract: An isolated monoclonal antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment is characterized by: (a) having a specific binding affinity to epithelial cell adhesion molecule (EpCAM) comprising the amino acid sequence of SEQ ID NO: 1; (b) having a specific binding affinity to cancer cells expressing EpCAM said cancer cells being selected from the group consisting of oral cancer cells, nasopharyngeal cancer cells (NPC), colorectal cancer cells, and ovarian cancer cells; and (c) having no binding affinity to human umbilical vein endothelial cell (HUVEC) and normal nasal mucosal epithelia (NNM). Also disclosed is an isolated monoclonal antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope within the sequence of KPEGALQNNDGLYDPDCDE (SEQ ID NO: 63) located within the EGF-like domain II of epithelial cell adhesion molecule (EpCAM). Methods of using the same are also disclosed.

Abstract translation: 公开了分离的单克隆抗体或其抗原结合片段。 抗体或抗原结合片段的特征在于：（a）对包含SEQ ID NO：1的氨基酸序列的上皮细胞粘附分子（EpCAM）具有特异性结合亲和力; （b）对表达EpCAM的癌细胞具有特异性结合亲和力，所述癌细胞选自口腔癌细胞，鼻咽癌细胞（NPC），结肠直肠癌细胞和卵巢癌细胞; 和（c）对人脐静脉内皮细胞（HUVEC）和正常鼻粘膜上皮细胞（NNM）没有结合亲和力。 还公开了与位于上皮细胞粘附分子（EpCAM）的EGF样结构域II内的KPEGALQNNDGLYDPDCDE（SEQ ID NO：63）的序列内的表位具有特异性结合亲和力的分离的单克隆抗体或其抗原结合片段 ）。 还公开了使用该方法的方法。

公开(公告)号：US09187558B2

公开(公告)日：2015-11-17

申请号：US14381362

申请日：2013-03-01

Applicant: ACADEMIA SINICA

Inventor： Han-Chung Wu ,  Mei-Ying Liao ,  Cheng-Wei Lin

IPC: A61K39/395 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574 ,  A61K39/00

CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/574

Abstract: An isolated monoclonal antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment is characterized by: (a) having a specific binding affinity to epithelial cell adhesion molecule (EpCAM) comprising the amino acid sequence of SEQ ID NO: 1; (b) having a specific binding affinity to cancer cells expressing EpCAM said cancer cells being selected from the group consisting of oral cancer cells, nasopharyngeal cancer cells (NPC), colorectal cancer cells, and ovarian cancer cells; and (c) having no binding affinity to human umbilical vein endothelial cell (HUVEC) and normal nasal mucosal epithelia (NNM). Also disclosed is an isolated monoclonal antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope within the sequence of KPEGALQNNDGLYDPDCDE (SEQ ID NO: 63) located within the EGF-like domain II of epithelial cell adhesion molecule (EpCAM). Methods of using the same are also disclosed.

Abstract translation: 公开了分离的单克隆抗体或其抗原结合片段。 抗体或抗原结合片段的特征在于：（a）对包含SEQ ID NO：1的氨基酸序列的上皮细胞粘附分子（EpCAM）具有特异性结合亲和力; （b）对表达EpCAM的癌细胞具有特异性结合亲和力，所述癌细胞选自口腔癌细胞，鼻咽癌细胞（NPC），结肠直肠癌细胞和卵巢癌细胞; 和（c）对人脐静脉内皮细胞（HUVEC）和正常鼻粘膜上皮细胞（NNM）没有结合亲和力。 还公开了与位于上皮细胞粘附分子（EpCAM）的EGF样结构域II内的KPEGALQNNDGLYDPDCDE（SEQ ID NO：63）的序列内的表位具有特异性结合亲和力的分离的单克隆抗体或其抗原结合片段 ）。 还公开了使用该方法的方法。