公开(公告)号：US20190177435A1

公开(公告)日：2019-06-13

申请号：US16184203

申请日：2018-11-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/44 ,  C07K16/30 ,  G01N33/574 ,  C07K16/18

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to globo H, SSEA3, and SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, skin, bone, lungs, breast, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervical, ovarian, and/or prostate cancer.

公开(公告)号：US09982041B2

公开(公告)日：2018-05-29

申请号：US14599174

申请日：2015-01-16

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/18 ,  G01N33/574 ,  C07K16/30 ,  A61K39/00

CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/734 ,  C07K2317/92 ,  G01N33/57407 ,  G01N33/57492 ,  G01N2800/52

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, lung, breast, mouse, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervix, ovary, and/or prostate cancer.

公开(公告)号：US20230366879A1

公开(公告)日：2023-11-16

申请号：US18308531

申请日：2023-04-27

Applicant: Academia Sinica

Inventor： Ying-Chih Chang ,  Han-Chung Wu ,  Po-Yuan Tseng ,  Jen-Chia Wu

IPC: G01N33/543 ,  C07K16/30 ,  G01N33/574 ,  C07K17/14 ,  G01N1/40

CPC classification number: G01N33/54393 ,  C07K16/30 ,  C07K17/14 ,  G01N1/405 ,  G01N33/54386 ,  G01N33/57492

Abstract: This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

公开(公告)号：US11674958B2

公开(公告)日：2023-06-13

申请号：US16832396

申请日：2020-03-27

Applicant: Academia Sinica

Inventor： Ying-Chih Chang ,  Han-Chung Wu ,  Po-Yuan Tseng ,  Jen-Chia Wu

IPC: G01N33/543 ,  G01N33/574 ,  G01N1/40 ,  C07K17/14 ,  C07K16/30

CPC classification number: G01N33/54386 ,  C07K16/30 ,  C07K17/14 ,  G01N1/405 ,  G01N33/54393 ,  G01N33/57492

Abstract: This invention relates to a surface coating for capture circulating rare cells, comprising a nonfouling composition to prevent the binding of non-specific cells and adsorption of serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the nonfouling and bioactive compositions. The invention also provide a surface coating for capture and purification of a biological substance, comprising a releasable composition to release the non-specific cells and other serum components; a bioactive composition for binding the biological substance, such as circulating tumor cells; with or without a linker composition that binds the releasable and bioactive compositions. The present invention also discloses a novel microfluidic chip, with specific patterned microstructures to create a flow disturbance and increase the capture rate of the biological substance.

公开(公告)号：US10196447B2

公开(公告)日：2019-02-05

申请号：US15563950

申请日：2016-04-12

Applicant: Academia Sinica

Inventor： Han-Chung Wu ,  Ruei-Min Lu ,  Chiung-Yi Chiu ,  I-Ju Liu ,  Yu-Ling Chang

IPC: A61K39/00 ,  A61P35/00 ,  A61P35/02 ,  C07K16/22 ,  C07K16/28 ,  C07K16/30 ,  A61K31/337 ,  A61K39/395 ,  C12N15/113 ,  G01N33/574

Abstract: An isolated antibody or an antigen-binding fragment thereof having a specific binding affinity to an epitope located within the domain 1 or domain 3 of human vascular endothelial growth factor receptor 2 (VEGFR2; SEQ ID NO: 74) is disclosed. The epitope within the domain 3 of the VEGFR2 is located between amino acid residues 250 and 270 of SEQ ID NO: 74. Use of the antibody or antigen-binding fragment thereof in the manufacture of a medicament for inhibiting tumor growth, tumor angiogenesis, and/or inducing cancer cell cytotoxicity in a subject in need thereof is also disclosed. Also disclosed is a method of detecting the presence of VEGFR2 in a tumor vascular endothelial cell or a cancer cell in a biological sample.

公开(公告)号：US10150818B2

公开(公告)日：2018-12-11

申请号：US14798312

申请日：2015-07-13

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/44 ,  G01N33/574 ,  C07K16/30 ,  C07K16/18 ,  A61K39/00

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to globo H, SSEA3, and SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, skin, bone, lungs, breast, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervical, ovarian, and/or prostate cancer.

公开(公告)号：US09975965B2

公开(公告)日：2018-05-22

申请号：US15011543

申请日：2016-01-30

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/44 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574 ,  A61K39/00

CPC classification number: C07K16/44 ,  A61K2039/505 ,  C07K16/18 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  G01N33/57407 ,  G01N2800/52

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to globo H, SSEA3, and SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, skin, bone, lungs, breast, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervical, ovarian, and/or prostate cancer.

公开(公告)号：US20150017230A1

公开(公告)日：2015-01-15

申请号：US14381362

申请日：2013-03-01

Applicant: ACADEMIA SINICA

Inventor： Han-Chung Wu ,  Mei-Ying Liao ,  Cheng-Wei Lin

IPC: C07K16/18 ,  G01N33/574

CPC classification number: C07K16/18 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/574

Abstract: An isolated monoclonal antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment is characterized by: (a) having a specific binding affinity to epithelial cell adhesion molecule (EpCAM) comprising the amino acid sequence of SEQ ID NO: 1; (b) having a specific binding affinity to cancer cells expressing EpCAM said cancer cells being selected from the group consisting of oral cancer cells, nasopharyngeal cancer cells (NPC), colorectal cancer cells, and ovarian cancer cells; and (c) having no binding affinity to human umbilical vein endothelial cell (HUVEC) and normal nasal mucosal epithelia (NNM). Also disclosed is an isolated monoclonal antibody or an antigen-binding fragment thereof that has a specific binding affinity to an epitope within the sequence of KPEGALQNNDGLYDPDCDE (SEQ ID NO: 63) located within the EGF-like domain II of epithelial cell adhesion molecule (EpCAM). Methods of using the same are also disclosed.

Abstract translation: 公开了分离的单克隆抗体或其抗原结合片段。 抗体或抗原结合片段的特征在于：（a）对包含SEQ ID NO：1的氨基酸序列的上皮细胞粘附分子（EpCAM）具有特异性结合亲和力; （b）对表达EpCAM的癌细胞具有特异性结合亲和力，所述癌细胞选自口腔癌细胞，鼻咽癌细胞（NPC），结肠直肠癌细胞和卵巢癌细胞; 和（c）对人脐静脉内皮细胞（HUVEC）和正常鼻粘膜上皮细胞（NNM）没有结合亲和力。 还公开了与位于上皮细胞粘附分子（EpCAM）的EGF样结构域II内的KPEGALQNNDGLYDPDCDE（SEQ ID NO：63）的序列内的表位具有特异性结合亲和力的分离的单克隆抗体或其抗原结合片段 ）。 还公开了使用该方法的方法。

公开(公告)号：US11524978B2

公开(公告)日：2022-12-13

申请号：US16823356

申请日：2020-03-19

Applicant: Academia Sinica

Inventor： Han-Chung Wu ,  Yuan-Sung Kuo

IPC: C07K7/06 ,  A61K47/64 ,  A61K38/43

Abstract: Disclosed herein is a biomolecule comprising a synthetic peptide for targeting thrombus, a pharmaceutical composition comprising the same, and uses thereof in the treatment of thromboembolism-related diseases. According to embodiments of the present disclosure, the synthetic peptide having the amino acid sequence of SEQ ID NO: 1, 2, or 3 exhibits a binding affinity and specificity to thrombus. Thus, the synthetic peptide may serve as a targeting element for delivering a therapeutic agent (e.g., an anticoagulant agent or a thrombolytic agent) to the thrombus thereby improving the therapeutic safety and efficacy of the therapeutic agent.

公开(公告)号：US11357862B2

公开(公告)日：2022-06-14

申请号：US16062274

申请日：2017-01-11

Applicant: Academia Sinica

Inventor： Han-Chung Wu ,  Chen-Yun Yeh

IPC: A61K47/62 ,  A61K47/69 ,  A61K47/66 ,  A61P35/00 ,  A61K9/127 ,  A61K49/00 ,  A61K49/18 ,  C07K7/08 ,  A61K47/64

Abstract: An isolated or a synthetic targeting peptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 8 is disclosed. The targeting peptide may be conjugated to a component selected from the group consisting of polymeric micelles, lipoprotein-based drug carriers, nanoparticle drug carriers, a chemotherapeutic agent, a micelle, a liposome, dendrimers, a polymer, a lipid, an oligonucleotide, a peptide, a polypeptide, a protein, a prostate cancer cell, a stem cell, and an imaging agent. Also disclosed are a kit for imaging and detecting the presence of prostate cancer cells in vivo or in vitro, and a composition for treating prostate cancer, inhibiting prostate cancer cell growth, inducing prostate cancer cell cytotoxicity, and/or increasing the survival rate in a prostate cancer patient.