公开(公告)号：US20140142157A1

公开(公告)日：2014-05-22

申请号：US13929444

申请日：2013-06-27

Applicant: ACADEMIA SINICA ,  JIN XIN BIOTECHNOLOGY CO., LTD.

Inventor： Kun-Hsuan HUANG ,  Tza-Zen CHANG ,  Pei-Wen HSIAO ,  Chia-Jui TSAI

IPC: A61K36/12 ,  A61K31/232 ,  A61K31/231 ,  A61K31/409

CPC classification number: A61K36/12 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/23 ,  A61K31/231 ,  A61K31/232 ,  A61K31/409 ,  A23V2200/30

Abstract: An extract of Asplenium nidus L. includes pyropheophorbide a methyl ester (C34H36N4O3), pheophorbide a methyl ester (C35H36N4O5), 1-linleoyl-3-linolenoyl-glycerol (C39H66O5), 1-linleoyl-2,3-dipalmitoyl-rac-glycerol (C53H98O6) and 1,3-dipalmitoyl-sn-glycerol (C35H68O5). The extract of Asplenium nidus L. is obtained by using a method including steps of: solvent extraction, using an solvent to extract an Asplenium nidus L. sample and to obtain an extract, with a w/v ratio between the solvent and the Asplenium nidus L. sample being 50-60 mg/ml; and column chromatography, fractionating the extract with water and ethanol as eluent to obtain several fractions including fraction a to fraction i, and further evaporating and lyophilizing fraction b, c, d or their combination to obtain an extract of Asplenium nidus L.

Abstract translation: of藜提取物包括焦脱镁叶酸甲酯（C34H36N4O3），偏硼酸甲酯（C35H36N4O5），1-亚麻酰-3-亚油酸 - 甘油（C39H66O5），1-亚麻酰基-2,3-二棕榈酰基 - 外消旋甘油 （C53H98O6）和1,3-二棕榈酰基-sn-甘油（C35H68O5）。 通过使用以下方法获得of The提取物，该方法包括以下步骤：溶剂萃取，使用溶剂萃取an an L. L. L. L. L. and and and and L L L L L L L L L L L L L 样品为50-60 mg / ml; 和柱色谱法，用水和乙醇作为洗脱液将提取物分馏，得到几种级分，包括级分a至级分i，并进一步蒸发和冻干级分b，c，d或其组合，得到As an提取物。