公开(公告)号：US20190300851A1

公开(公告)日：2019-10-03

申请号：US16302615

申请日：2017-05-16

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Christine CHEUNG ,  Balakrishnan Chakrapani NARMADA ,  Yeek Teck GOH

IPC: C12N5/071 ,  G01N33/50

Abstract: The present disclosure provides a method of deriving endothelial cells, comprising (a) culturing lateral plate mesoderm cells under oxygen-deprived condition to obtain endothelial lineage cells; and (b) culturing endothelial cells from (a) on an extracellular matrix to maintain and expand the endothelial lineage cells. Also disclosed herein is a cell co-culture system comprising an endothelial cell culture and a hepatocyte cell culture, as well as a microfluidic-based system comprising said cell co-culture system. Also disclosed herein is a method of disease modelling or drug testing using said cell co-culture system or said microfluidic-based system.