公开(公告)号：US11414681B2

公开(公告)日：2022-08-16

申请号：US16430410

申请日：2019-06-03

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Jia Wei Siau ,  Peter Droge ,  Harshyaa Makhija ,  Shree Harsh Vijaya Chandra ,  Sabrina Peter

IPC: C12N9/12 ,  C12N9/22 ,  C12N1/15 ,  C12N1/21 ,  C12P19/34 ,  C12N15/90

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

公开(公告)号：US10344301B2

公开(公告)日：2019-07-09

申请号：US15502783

申请日：2015-08-11

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Jia Wei Siau ,  Peter Droge ,  Harshyaa Makhija ,  Shree Harsh Vijaya Chandra ,  Sabrina Peter

IPC: C12N9/12 ,  C12N9/22 ,  C12N1/15 ,  C12N1/21 ,  C12P19/34 ,  C12N15/90

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.