Methods to identify protein interaction

    公开(公告)号:US11630110B2

    公开(公告)日:2023-04-18

    申请号:US16632646

    申请日:2018-08-20

    Inventor: Soon Heng Tan

    Abstract: The present invention discloses methods for identifying a protein interaction between one or more first proteins and one or more further proteins comprising the steps of: exposing one or more samples comprising the proteins to at least one preselected condition for at least one preselected duration; isolating and separating at least one soluble fraction from an insoluble fraction of said one or more samples; and analyzing the at least one soluble fraction or the insoluble fraction to identify said protein interaction between one or more first proteins and one or more further proteins. Use of the method of the invention are also disclosed.

    METHODS FOR IDENTIFYING AND MONITORING INTERACTIONS OF PROTEIN WITH LIGAND

    公开(公告)号:US20240302379A1

    公开(公告)日:2024-09-12

    申请号:US18258775

    申请日:2021-12-20

    Inventor: Soon Heng Tan

    CPC classification number: G01N33/6803 G01N2800/52

    Abstract: The invention relates generally to the field of biochemistry. In particular, the invention relates to a method of detecting or measuring target that is bound to a ligand in a sample, the method comprising contacting a sample comprising one or more cells with a cell-permeable denaturant to promote intracellular unfolding of the target in the sample, followed by lysing the sample. The lysed sample is then detected or measured for the level of non-aggregated target or aggregated target, wherein a difference in the level of non-aggregated target or aggregated target as compared to a reference indicates the presence or level of target that is bound to the ligand in the sample. In specific embodiments, the cell-permeable denaturant is urea or derivatives thereof. Methods of identifying a candidate ligand or predicting the efficacy of a drug in a subject are also provided therein.

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